ArticleBMC genomics2008
Codon usage suggests that translational selection has a major impact on protein expression in trypanosomatids.
Article in BMC genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- Host mature tRNAome as a decoding switch regulates antiviral and proviral responses.Nature communications · 2026Article
- Tetracycline-Inducible Functional Complementation Analysis in Trypanosoma Parasites.Methods in molecular biology (Clifton, N.J.) · 2026Article
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- Decoding post-transcriptional gene expression controls in trypanosomatids using machine learning.Wellcome open research · 2025Article
- Lack of transcriptional coordination between mitochondrial and nuclear oxidative phosphorylation genes in the presence of two divergent mitochondrial genomes.Zoological research · 2022Article
- Ionizing radiation and chemical oxidant exposure impacts on Cryptococcus neoformans transfer RNAs.PloS one · 2022Article
- Factors affecting the rapid changes of protein under short-term heat stress.BMC genomics · 2021Article
- tRNA 3' shortening by LCCR4 as a response to stress in Trypanosoma brucei.Nucleic acids research · 2021Article
- Natural Selection Plays an Important Role in Shaping the Codon Usage of Structural Genes of the Viruses Belonging to theViruses · 2020Article
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- A global analysis of low-complexity regions in theWellcome open research · 2020Article
- Enhancement of Expression Level of Modified t-PA (TNKase) in Leishmania tarentolae by Induction SystemIranian biomedical journal · 2019Article
- Evolution of Antigenic Variation in African Trypanosomes: Variant Surface Glycoprotein Expression, Structure, and Function.BioEssays : news and reviews in molecular, cellular and developmental biology · 2018Review
- Exploiting CRISPR-Cas9 technology to investigate individual histone modifications.Nucleic acids research · 2018Article
- Selection-driven cost-efficiency optimization of transcripts modulates gene evolutionary rate in bacteria.Genome biology · 2018Article
- Independent evolution of functionally exchangeable mitochondrial outer membrane import complexes.eLife · 2018Article
- Inducible high-efficiency CRISPR-Cas9-targeted gene editing and precision base editing in African trypanosomes.Scientific reports · 2018Article
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Abstract
backgroundDifferent proteins are required in widely different quantities to build a living cell. In most organisms, transcription control makes a major contribution to differential expression. This is not the case in trypanosomatids where most genes are transcribed at an equivalent rate within large polycistronic clusters. Thus, trypanosomatids must use post-transcriptional control mechanisms to balance gene expression requirements.
resultsHere, the evidence for translational selection, the enrichment of 'favoured' codons in more highly expressed genes, is explored. A set of highly expressed, tandem-repeated genes display codon bias in Trypanosoma cruzi, Trypanosoma brucei and Leishmania major. The tRNA complement reveals forty-five of the sixty-one possible anticodons indicating widespread use of 'wobble' tRNAs. Consistent with translational selection, cognate tRNA genes for favoured codons are over-represented. Importantly, codon usage (Codon Adaptation Index) correlates with predicted and observed expression level. In addition, relative codon bias is broadly conserved among syntenic genes from different trypanosomatids.
conclusionSynonymous codon bias is correlated with tRNA gene copy number and with protein expression level in trypanosomatids. Taken together, the results suggest that translational selection is the dominant mechanism underlying the control of differential protein expression in these organisms. The findings reveal how trypanosomatids may compensate for a paucity of canonical Pol II promoters and subsequent widespread constitutive RNA polymerase II transcription.
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