ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2008
Heterogeneity in synaptic vesicle release at neuromuscular synapses of mice expressing synaptopHluorin.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 42 citations in OpenAlex.
- Frequency-Dependent Engagement of Synaptic Vesicle Pools in the Mice Motor Nerve Terminals.Cellular and molecular neurobiology · 2023Article
- Spontaneously Recycling Synaptic Vesicles Constitute Readily Releasable Vesicles in Intact Neuromuscular Synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Similarity and Diversity of Presynaptic Molecules at Neuromuscular Junctions and Central Synapses.Biomolecules · 2022Review
- Brain derived neurotrophic factor/tropomyosin related kinase B signaling impacts diaphragm neuromuscular transmission in a novel rat chemogenetic model.Frontiers in cellular neuroscience · 2022Article
- Methods of measuring presynaptic function with fluorescence probes.Applied microscopy · 2021Review
- Properties of Glial Cell at the Neuromuscular Junction Are Incompatible with Synaptic Repair in theThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Presynaptic active zones of mammalian neuromuscular junctions: Nanoarchitecture and selective impairments in aging.Neuroscience research · 2018Review
- Impact of spatiotemporal calcium dynamics within presynaptic active zones on synaptic delay at the frog neuromuscular junction.Journal of neurophysiology · 2018Article
- Homeostatic synaptic plasticity at the neuromuscular junction in myasthenia gravis.Annals of the New York Academy of Sciences · 2018Review
- Functionally heterogeneous synaptic vesicle pools support diverse synaptic signalling.The Journal of physiology · 2016Review
- Different dynamin blockers interfere with distinct phases of synaptic endocytosis during stimulation in motoneurones.The Journal of physiology · 2015Article
- TIRFM and pH-sensitive GFP-probes to evaluate neurotransmitter vesicle dynamics in SH-SY5Y neuroblastoma cells: cell imaging and data analysis.Journal of visualized experiments : JoVE · 2015Article
- An assay to image neuronal microtubule dynamics in mice.Nature communications · 2014Article
- Organization and function of transmitter release sites at the neuromuscular junction.The Journal of physiology · 2013Review
- Evaluation of a novel calcium channel agonist for therapeutic potential in Lambert-Eaton myasthenic syndrome.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Structural and functional maturation of active zones in large synapses.Molecular neurobiology · 2013Review
- Synaptic vesicle pools and dynamics.Cold Spring Harbor perspectives in biology · 2012Review
- Molecular mechanism of active zone organization at vertebrate neuromuscular junctions.Molecular neurobiology · 2012Review
- Presynaptic Active Zone Density during Development and Synaptic Plasticity.Frontiers in molecular neuroscience · 2012Article
- Design and application of genetically encoded biosensors.Trends in biotechnology · 2011Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Mammalian neuromuscular junctions are useful model synapses to study the relationship between synaptic structure and function, although these have rarely been studied together at the same synapses. To do this, we generated transgenic lines of mice in which the thy1.2 promoter drives expression of synaptopHluorin (spH) as a means of optically measuring synaptic vesicle distribution and release. SpH is colocalized with other synaptic vesicle proteins in presynaptic terminals and does not alter normal synaptic function. Nerve stimulation leads to readily detectable and reproducible fluorescence changes in motor axon terminals that vary with stimulus frequency and, when compared with electrophysiological recordings, are reliable indicators of neurotransmitter release. Measurements of fluorescence intensity changes reveal a surprising amount of heterogeneity in synaptic vesicle release throughout individual presynaptic motor axon terminals. Some discrete terminal regions consistently displayed a greater rate and extent of release than others, regardless of stimulation frequency. The amount of release at a particular site is highly correlated to the relative abundance of synaptic vesicles there, indicating that a relatively constant fraction of the total vesicular pool, approximately 30%, is released in response to activity. These studies reveal previously unknown relationships between synaptic structure and function at mammalian neuromuscular junctions and demonstrate the usefulness of spH expressing mice as a tool for studying neuromuscular synapses in adults, as well as during development and diseases that affect neuromuscular synaptic function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.