ArticleThe Journal of biological chemistry2008
General transcription factor IIA-gamma increases osteoblast-specific osteocalcin gene expression via activating transcription factor 4 and runt-related transcription factor 2.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Critical role of AKT protein in myeloma-induced osteoclast formation and osteolysis.The Journal of biological chemistry · 2013Trial
- Role of ATF7-TAF12 interactions in the vitamin D response hypersensitivity of osteoclast precursors in Paget's disease.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2013Trial
- Osteoarthritis: pathogenic signaling pathways and therapeutic targets.Signal transduction and targeted therapy · 2023Review
- Runx2-interacting genes identified by yeast two-hybrid screening of libraries generated from hypertrophic chondrocytes.American journal of translational research · 2016Article
- Article
- ATF4 promotes bone angiogenesis by increasing VEGF expression and release in the bone environment.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2013Article
- Article
- atf4 promotes β-catenin expression and osteoblastic differentiation of bone marrow mesenchymal stem cells.International journal of biological sciences · 2013Article
- PTHrP expression in human MDA-MB-231 breast cancer cells is critical for tumor growth and survival and osteoblast inhibition.International journal of biological sciences · 2013Article
- Critical role of filamin-binding LIM protein 1 (FBLP-1)/migfilin in regulation of bone remodeling.The Journal of biological chemistry · 2012Article
- Combinatorial control of ATF4-dependent gene transcription in osteoblasts.Annals of the New York Academy of Sciences · 2011Review
- Foxo1 mediates insulin-like growth factor 1 (IGF1)/insulin regulation of osteocalcin expression by antagonizing Runx2 in osteoblasts.The Journal of biological chemistry · 2011Article
- MAPK phosphatase-3 promotes hepatic gluconeogenesis through dephosphorylation of forkhead box O1 in mice.The Journal of clinical investigation · 2010Article
- Activating transcription factor 4 regulates osteoclast differentiation in mice.The Journal of clinical investigation · 2010Article
- Critical role of activating transcription factor 4 in the anabolic actions of parathyroid hormone in bone.PloS one · 2009Article
- CCAAT/enhancer-binding protein beta promotes osteoblast differentiation by enhancing Runx2 activity with ATF4.Molecular biology of the cell · 2008Article
- Activating transcription factor 4 is critical for proliferation and survival in primary bone marrow stromal cells and calvarial osteoblasts.Journal of cellular biochemistry · 2008Article
- Activated transcription via mammalian amino acid response elements does not require enhanced recruitment of the Mediator complex.Nucleic acids research · 2008Article
- Regulation of gene expression in osteoblasts.BioFactors (Oxford, England)Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
ATF4 (activating transcription factor 4) is an osteoblast-enriched transcription factor that regulates terminal osteoblast differentiation and bone formation. ATF4 knock-out mice have reduced bone mass (severe osteoporosis) throughout life. Runx2 (runt-related transcription factor 2) is a runt domain-containing transcription factor that is essential for bone formation during embryogenesis and postnatal life. In this study, we identified general transcription factor IIA gamma (TFIIA gamma) as a Runx2-interacting factor in a yeast two-hybrid screen. Immunoprecipitation assays confirmed that TFIIA gamma interacts with Runx2 in osteoblasts and when coexpressed in COS-7 cells or using purified glutathione S-transferase fusion proteins. Chromatin immunoprecipitation assay of MC3T3-E1 (clone MC-4) preosteoblast cells showed that in intact cells TFIIA gamma is recruited to the region of the osteocalcin promoter previously shown to bind Runx2 and ATF4. A small region of Runx2 (amino acids 258-286) was found to be required for TFIIA gamma binding. Although TFIIA gamma interacts with Runx2, it does not activate Runx2. Instead, TFIIA gamma binds to and activates ATF4. Furthermore, TFIIA gamma together with ATF4 and Runx2 stimulates osteocalcin promoter activity and endogenous mRNA expression. Small interfering RNA silencing of TFIIA gamma markedly reduces levels of endogenous ATF4 protein and Ocn mRNA in osteoblastic cells. Overexpression of TFIIA gamma increases levels of ATF4 protein. Finally, TFIIA gamma significantly prevents ATF4 degradation. This study shows that a general transcription factor, TFIIA gamma, facilitates osteoblast-specific gene expression through interactions with two important bone transcription factors ATF4 and Runx2.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.