ArticleJournal of biomolecular techniques : JBT2007
The methodology used to measure differential gene expression affects the outcome.
Article in Journal of biomolecular techniques : JBT, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- MEK4 function, genistein treatment, and invasion of human prostate cancer cells.Journal of the National Cancer Institute · 2009Trial
- Transcriptomic Approaches to Cardiomyocyte-Biomaterial Interactions: A Review.ACS biomaterials science & engineering · 2024Review
- Identification and validation of reference genes in vetiver (Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- PhyloM: A Computer Program for Phylogenetic Inference from Measurement or Binary Data, with Bootstrapping.Life (Basel, Switzerland) · 2022Article
- DNA nanotechnology approaches for microRNA detection and diagnosis.Nucleic acids research · 2019Review
- Mitochondrial aconitase is a key regulator of energy production for growth and protein expression in Chinese hamster ovary cells.Metabolomics : Official journal of the Metabolomic Society · 2018Article
- Precision therapeutic targeting of human cancer cell motility.Nature communications · 2018Article
- Mitogen-activated protein kinase kinase 4 (MAP2K4) promotes human prostate cancer metastasis.PloS one · 2014Article
- Gene expression profiling for the purposes of biomarker discovery in oral potentially malignant lesions: a systematic review.Clinical Medicine Insights. Oncology · 2013Review
- Transcriptional regulatory circuitries in the human pathogen Candida albicans involving sense--antisense interactions.Genetics · 2012Article
- WWOX expression in colorectal cancer--a real-time quantitative RT-PCR study.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2011Article
- Endoglin suppresses human prostate cancer metastasis.Clinical & experimental metastasis · 2011Article
- Article
- Cellular processes of v-Src transformation revealed by gene profiling of primary cells--implications for human cancer.BMC cancer · 2010Article
- Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Confirmation of gene expression by a second methodology is critical in order to detect false-positive findings associated with microarrays. However, the impact of methodology upon the measurement of gene expression has not been rigorously evaluated. In the current study, we compared differential gene expression between PC3 and PC3-M human prostate cancer cell lines using three separate methods: microarray, quantitative RT/PCR (qRT/PCR), and Northern blotting. The PC3 to PC3-M ratio of gene expression was determined for each of 24 different genes evaluated, by each of the three methods. Comparison of gene expression ratios between Northern and microarray, Northern and qRT/PCR, and microarray and qRT/PCR, gave correlation coefficients (r) of 0.72, 0.39, and 0.63, respectively. In each instance, one to two outlier genes were apparent. Their exclusion from analysis gave r values of 0.79, 0.72, and 0.83, respectively. These findings demonstrate that the assessment of differential gene expression is dependent upon the methodology used in each situation where outcome between different methodologies was compared, the presence of a relatively limited number of outlier genes precludes high overall correlation between the methods. Validation of gene expression by different methods should be performed whenever possible.
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18166675PMC2392989What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.