ArticleThe Journal of cell biology2007
Two translocating hydrophilic segments of a nascent chain span the ER membrane during multispanning protein topogenesis.
Article in The Journal of cell biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 47 citations in OpenAlex.
- Interaction mapping of the Sec61 translocon identifies two Sec61α regions interacting with hydrophobic segments in translocating chains.The Journal of biological chemistry · 2018Article
- The Ribosome-Sec61 Translocon Complex Forms a Cytosolically Restricted Environment for Early Polytopic Membrane Protein Folding.The Journal of biological chemistry · 2015Article
- Marginally hydrophobic transmembrane α-helices shaping membrane protein folding.Protein science : a publication of the Protein Society · 2015Review
- Lipids and topological rules governing membrane protein assembly.Biochimica et biophysica acta · 2014Review
- Transmembrane segments form tertiary hairpins in the folding vestibule of the ribosome.Journal of molecular biology · 2014Article
- Membrane translocation of lumenal domains of membrane proteins powered by downstream transmembrane sequences.Molecular biology of the cell · 2013Article
- In vitro reconstitution of lipid-dependent dual topology and postassembly topological switching of a membrane protein.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Membrane protein TM segments are retained at the translocon during integration until the nascent chain cues FRET-detected release into bulk lipid.Molecular cell · 2012Article
- Lipid-dependent generation of dual topology for a membrane protein.The Journal of biological chemistry · 2012Article
- Mechanisms for quality control of misfolded transmembrane proteins.Biochimica et biophysica acta · 2012Review
- Yos9p and Hrd1p mediate ER retention of misfolded proteins for ER-associated degradation.Molecular biology of the cell · 2012Article
- Role of human sec63 in modulating the steady-state levels of multi-spanning membrane proteins.PloS one · 2012Article
- Transmembrane segments of nascent polytopic membrane proteins control cytosol/ER targeting during membrane integration.The Journal of cell biology · 2011Article
- Article
- Positive charges of translocating polypeptide chain retrieve an upstream marginal hydrophobic segment from the endoplasmic reticulum lumen to the translocon.Molecular biology of the cell · 2010Article
- Environmental transition of signal-anchor sequences during membrane insertion via the endoplasmic reticulum translocon.Molecular biology of the cell · 2010Article
- Cellular mechanisms of membrane protein folding.Nature structural & molecular biology · 2009Article
- Visualization of distinct entities of the SecYEG translocon during translocation and integration of bacterial proteins.Molecular biology of the cell · 2009Article
- Lipid-dependent membrane protein topogenesis.Annual review of biochemistry · 2009Review
- Sequence-specific retention and regulated integration of a nascent membrane protein by the endoplasmic reticulum Sec61 translocon.Molecular biology of the cell · 2009Article
Corrections and comments
- Commented on by
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During protein integration into the endoplasmic reticulum, the N-terminal domain preceding the type I signal-anchor sequence is translocated through a translocon. By fusing a streptavidin-binding peptide tag to the N terminus, we created integration intermediates of multispanning membrane proteins. In a cell-free system, N-terminal domain (N-domain) translocation was arrested by streptavidin and resumed by biotin. Even when N-domain translocation was arrested, the second hydrophobic segment mediated translocation of the downstream hydrophilic segment. In one of the defined intermediates, two hydrophilic segments and two hydrophobic segments formed a transmembrane disposition in a productive state. Both of the translocating hydrophilic segments were crosslinked with a translocon subunit, Sec61alpha. We conclude that two translocating hydrophilic segment in a single membrane protein can span the membrane during multispanning topogenesis flanking the translocon. Furthermore, even after six successive hydrophobic segments entered the translocon, N-domain translocation could be induced to restart from an arrested state. These observations indicate the remarkably flexible nature of the translocon.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.