ArticleThrombosis research2008
Regulated complement deposition on the surface of human endothelial cells: effect of tobacco smoke and shear stress.
Article in Thrombosis research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- Impact of smoking on the complement system: a narrative review.Frontiers in immunology · 2025Review
- Eculizumab Use in Scleroderma Renal Crisis With Thrombotic Microangiopathy: A Case Report.Kidney medicine · 2024Article
- PepO and CppA modulateVirulence · 2023Article
- gC1qR Antibody Can Modulate Endothelial Cell Permeability in Angioedema.Inflammation · 2022Article
- Diabetes and other vascular risk factors in association with the risk of lower extremity amputation in chronic limb-threatening ischemia: a prospective cohort study.Cardiovascular diabetology · 2022Article
- Functional genetic variants in complement component 7 confer susceptibility to gastric cancer.PeerJ · 2022Article
- Diversity in Phenotypes Associated With Host Persistence and Systemic Virulence inFrontiers in microbiology · 2022Article
- Antirheumatic therapy is associated with reduced complement activation in rheumatoid arthritis.PloS one · 2022Article
- Review
- Impact of Tobacco Smoke in HIV Progression: a Major Risk Factor for the Development of NeuroAIDS and Associated of CNS Disorders.Zeitschrift fur Gesundheitswissenschaften = Journal of public health · 2020Article
- Clinical significance of C4d deposition in renal tissues from patients with primary Sjögren's syndrome-a preliminary study.BMC nephrology · 2019Article
- P-selectin drives complement attack on endothelium during intravascular hemolysis in TLR-4/heme-dependent manner.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Tumor necrosis factor inhibitors are associated with reduced complement activation in spondylarthropathies: An observational study.PloS one · 2019Observational
- Mother and Child Reunion in "Hypertensive" End-Stage Renal Disease: Will They Complement Each Other?Nephron · 2019Article
- C5b9 Formation on Endothelial Cells Reflects Complement Defects among Patients with Renal Thrombotic Microangiopathy and Severe Hypertension.Journal of the American Society of Nephrology : JASN · 2018Article
- Endothelial Cell Inflammatory Reactions Are Altered in the Presence of E-Cigarette Extracts of Variable Nicotine.Cellular and molecular bioengineering · 2017Article
- A common CD55 rs2564978 variant is associated with the susceptibility of non-small cell lung cancer.Oncotarget · 2017Article
- Article
- The Risk Factors for Failure of an Upper Extremity Replantation: Is the Use of Cigarettes/Tobacco a Significant Factor?PloS one · 2015Article
- Smoke exposure causes endoplasmic reticulum stress and lipid accumulation in retinal pigment epithelium through oxidative stress and complement activation.The Journal of biological chemistry · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Cigarette smoke and hemodynamic stress both contribute to vascular inflammation and associated atherosclerosis. We recently demonstrated direct activation of complement components C4 and C3 on human endothelial cells (EC). The present study was designed to explore complement activation on bone marrow microvascular endothelial cells (BMEC) and human umbilical vein endothelial cells (HUVEC) in response to endothelial cell injury by tobacco smoke extract, shear stress, or other known inflammatory and atherogenic mediators, lipopolysaccharide (LPS) and INF-gamma. Following treatment, confluent EC monolayers were exposed to plasma (60 min, 37 degrees C), and cell surface deposition of stable complement derivatives C4d, iC3b and SC5b-9 was measured in situ using an ELISA approach. Consistent with previous results, moderate levels of C4d, iC3b and SC5b-9 deposition were observed on native EC monolayers exposed to human plasma. Tobacco smoke and shear stress enhanced EC C4d deposition. In contrast, LPS and INF-gamma failed to affect EC mediated complement activation, despite evidence of EC activation illustrated by ICAM-1 expression. The combination of tobacco smoke and shear stress nearly doubled EC C4d expression. No increases in iC3b or SC5b-9 were noted, suggesting inhibition of classical and alternative pathway C3 convertase assembly or activity. Indeed, concomitantly increased surface expression of complement regulatory proteins CD35 (CR1) and CD55 was observed following EC exposure to tobacco smoke and shear stress. These results suggest that a balance between complement activation and regulation exists at the EC surface, and may impact vascular injury leading to thrombosis, arteriosclerosis, and atherogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.