ArticleJournal of molecular biology2008
The three-dimensional structure of CFA/I adhesion pili: traveler's diarrhea bacteria hang on by a spring.
Article in Journal of molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- Impact of an alpha helix and a cysteine-cysteine disulfide bond on the resistance of bacterial adhesion pili to stress.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogenesis.PLoS pathogens · 2020Article
- Cryo-EM structure of the CFA/I pilus rod.IUCrJ · 2019Article
- A Role for Salivary Peptides in the Innate Defense Against Enterotoxigenic Escherichia coli.The Journal of infectious diseases · 2018Article
- Genome-Wide Identification by Transposon Insertion Sequencing of Escherichia coli K1 Genes Essential forJournal of bacteriology · 2018Article
- Providing Structure to Enterotoxigenic Escherichia coli Vaccine Development.The Journal of infectious diseases · 2017Article
- Binding of CFA/I Pili of Enterotoxigenic Escherichia coli to Asialo-GM1 Is Mediated by the Minor Pilin CfaE.Infection and immunity · 2016Article
- Antibody-mediated disruption of the mechanics of CS20 fimbriae of enterotoxigenic Escherichia coli.Scientific reports · 2015Article
- Structure and function of enterotoxigenic Escherichia coli fimbriae from differing assembly pathways.Molecular microbiology · 2015Article
- Contribution of the highly conserved EaeH surface protein to enterotoxigenic Escherichia coli pathogenesis.Infection and immunity · 2014Article
- Novel antigens for enterotoxigenic Escherichia coli vaccines.Expert review of vaccines · 2014Review
- Virulence regulons of enterotoxigenic Escherichia coli.Immunologic research · 2013Review
- Adhesion of Diarrheagenic Escherichia coli and Inhibition by Glycocompounds Engaged in the Mucosal Innate Immunity.Biology · 2013Article
- The structure of the CS1 pilus of enterotoxigenic Escherichia coli reveals structural polymorphism.Journal of bacteriology · 2013Article
- Biofilm formation and binding specificities of CFA/I, CFA/II and CS2 adhesions of enterotoxigenic Escherichia coli and Cfae-R181A mutant.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2012Article
- A structural basis for sustained bacterial adhesion: biomechanical properties of CFA/I pili.Journal of molecular biology · 2012Article
- Shear-enhanced binding of intestinal colonization factor antigen I of enterotoxigenic Escherichia coli.Molecular microbiology · 2010Article
- Molecular mechanisms of enterotoxigenic Escherichia coli infection.Microbes and infection · 2010Review
- Structure of CFA/I fimbriae from enterotoxigenic Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- CfaE tip mutations in enterotoxigenic Escherichia coli CFA/I fimbriae define critical human intestinal binding sites.Cellular microbiology · 2009Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
To survive the harsh environment of a churning intestinal tract, bacteria attach to the host epithelium via thin fibers called pili (or fimbriae). Enterotoxigenic Escherichia coli bacteria expressing colonization factor antigen I (CFA/I) pili and related pili are the most common known bacterial cause of diarrheal disease, including traveler's diarrhea. CFA/I pili, assembled via the alternate chaperone pathway, are essential for binding and colonization of the small bowel by these pathogenic bacteria. Herein, we elucidate unique structural features of CFA/I pili that appear to optimize their function as bacterial tethers in the intestinal tract. Using transmission electron microscopy of negatively stained samples in combination with iterative three-dimensional helical reconstruction methods for image processing, we determined the structure of the CFA/I pilus filament. Our results indicate that strong end-to-end protein interactions and weak interactions between the coils of a sturdy spring-like helix provide the combination of strength, stability, and flexibility required to sustain bacterial adhesion and incite intestinal disease. We propose that CFA/I pili behave like a spring to maintain attachment to the gut lining during vortex mixing and downward flow of the intestinal contents, thereby persisting long enough for these bacteria to colonize the host epithelium and cause enteric disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.