ArticleApplied and environmental microbiology2008
Marker removal in staphylococci via Cre recombinase and different lox sites.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 67 citations in OpenAlex.
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- Bacterial genome engineering using CRISPR-associated transposases.Nature protocols · 2024Review
- Bacterial genome engineering using CRISPR RNA-guided transposases.bioRxiv : the preprint server for biology · 2023Article
- A Novel Cre/International journal of molecular sciences · 2021Article
- Impact of the Histidine-Containing Phosphocarrier Protein HPr on Carbon Metabolism and Virulence inMicroorganisms · 2021Article
- Lox'd in translation: contradictions in the nomenclature surrounding common lox-site mutants and their implications in experiments.Microbiology (Reading, England) · 2021Article
- Article
- Floxed-Cassette Allelic Exchange Mutagenesis Enables Markerless Gene Deletion in Chlamydia trachomatis and Can Reverse Cassette-Induced Polar Effects.Journal of bacteriology · 2018Article
- Antibiotic Combinations That Enable One-Step, Targeted Mutagenesis of Chromosomal Genes.ACS infectious diseases · 2018Article
- Cre/lox-based multiple markerless gene disruption in the genome of the extreme thermophile Thermus thermophilus.Molecular genetics and genomics : MGG · 2018Article
- A Novel Vector for Construction of Markerless Multicopy Overexpression Transformants inFrontiers in microbiology · 2017Article
- The Peptidoglycan Pattern of Staphylococcus carnosus TM300-Detailed Analysis and Variations Due to Genetic and Metabolic Influences.Antibiotics (Basel, Switzerland) · 2016Article
- High Frequency and Diversity of Antimicrobial Activities Produced by Nasal Staphylococcus Strains against Bacterial Competitors.PLoS pathogens · 2016Article
- The νSaα Specific Lipoprotein Like Cluster (lpl) of S. aureus USA300 Contributes to Immune Stimulation and Invasion in Human Cells.PLoS pathogens · 2015Article
- Novel Routes for Improving Biocontrol Activity of Bacillus Based Bioinoculants.Frontiers in microbiology · 2015Review
- The MazEF Toxin-Antitoxin System Alters the β-Lactam Susceptibility of Staphylococcus aureus.PloS one · 2015Article
- Catabolite control protein E (CcpE) is a LysR-type transcriptional regulator of tricarboxylic acid cycle activity in Staphylococcus aureus.The Journal of biological chemistry · 2013Article
- The mevalonate auxotrophic mutant of Staphylococcus aureus can adapt to mevalonate depletion.Antimicrobial agents and chemotherapy · 2013Article
- Selective methanol or formate production during continuous CO₂ fermentation by the acetogen biocatalysts engineered via integration of synthetic pathways using Tn7-tool.World journal of microbiology & biotechnology · 2013Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Allelic replacement in staphylococci is frequently aided by antibiotic resistance markers that replace the gene(s) of interest. In multiply modified strains, the number of mutated genes usually correlates with the number of selection markers in the strain's chromosome. Site-specific recombination systems are capable of eliminating such markers, if they are flanked by recombinase recognition sites. In this study, a Cre-lox setting was established that allowed the efficient removal of resistance genes from the genomes of Staphylococcus carnosus and S. aureus. Two cassettes conferring resistance to erythromycin or kanamycin were flanked with wild-type or mutant lox sites, respectively, and used to delete single genes and an entire operon. After transformation of the cells with a newly constructed cre expression plasmid (pRAB1), genomic eviction of the resistance genes was observed in approximately one out of ten candidates analyzed and subsequently verified by PCR. Due to its thermosensitive origin of replication, the plasmid was then easily eliminated at nonpermissive temperatures. We anticipate that the system presented here will prove useful for generating markerless deletion mutants in staphylococci.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.