ArticleApplied and environmental microbiology2008
Direct identification of a bacterial manganese(II) oxidase, the multicopper oxidase MnxG, from spores of several different marine Bacillus species.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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55 citing papers in PubMed, 160 citations in OpenAlex.
- Comparative transcriptomics and scarless genome editing uncover a periplasmicApplied and environmental microbiology · 2026Article
- Biological contributions to manganese oxides in rock varnish at Murujuga (Western Australia).Scientific reports · 2026Article
- Biogenic Mn Oxide Generation and Mn(II) Removal by a Manganese Oxidizing Bacterium Bacillus sp. Strain M2.Applied biochemistry and biotechnology · 2024Article
- Advances in Research on Bacterial Oxidation of Mn(II): A Visualized Bibliometric Analysis Based on CiteSpace.Microorganisms · 2024Review
- Active prokaryotic and eukaryotic viral ecology across spatial scale in a deep-sea brine pool.ISME communications · 2024Article
- Changes in the Microbiome of Sugarcane (Life (Basel, Switzerland) · 2023Article
- Microbe-driven elemental cycling enables microbial adaptation to deep-sea ferromanganese nodule sediment fields.Microbiome · 2023Article
- A halophilicFrontiers in microbiology · 2023Article
- Manganese Pollution and Its Remediation: A Review of Biological Removal and Promising Combination Strategies.Microorganisms · 2022Review
- Mineralogical and Genomic Constraints on the Origin of Microbial Mn Oxide Formation in Complexed Microbial Community at the Terrestrial Hot Spring.Life (Basel, Switzerland) · 2022Article
- A soil-borne Mn(II)-oxidizing bacterium of Providencia sp. exploits a strategy of superoxide production coupled to hydrogen peroxide consumption to generate Mn oxides.Archives of microbiology · 2022Article
- Article
- Proteomic insights into Lysinibacillus sp.-mediated biosolubilization of manganese.Environmental science and pollution research international · 2021Article
- Genome analysis of Pseudomonas sp. OF001 and Rubrivivax sp. A210 suggests multicopper oxidases catalyze manganese oxidation required for cylindrospermopsin transformation.BMC genomics · 2021Article
- Bacteria-induced mineral precipitation: a mechanistic review.Microbiology (Reading, England) · 2021Review
- Manganese Stress Adaptation Mechanisms ofFrontiers in microbiology · 2021Article
- Manganese-contaminated groundwater treatment by novel bacterial isolates: kinetic study and mechanism analysis using synchrotron-based techniques.Scientific reports · 2020Article
- Manganese (II) removal from aqueous solutions byBiotechnology reports (Amsterdam, Netherlands) · 2020Article
- Mn(III) species formed by the multi-copper oxidase MnxG investigated by electron paramagnetic resonance spectroscopy.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2018Article
- Metagenomic insights into the microbial diversity in manganese-contaminated mine tailings and their role in biogeochemical cycling of manganese.Scientific reports · 2018Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Microorganisms catalyze the formation of naturally occurring Mn oxides, but little is known about the biochemical mechanisms of this important biogeochemical process. We used tandem mass spectrometry to directly analyze the Mn(II)-oxidizing enzyme from marine Bacillus spores, identified as an Mn oxide band with an in-gel activity assay. Nine distinct peptides recovered from the Mn oxide band of two Bacillus species were unique to the multicopper oxidase MnxG, and one peptide was from the small hydrophobic protein MnxF. No other proteins were detected in the Mn oxide band, indicating that MnxG (or a MnxF/G complex) directly catalyzes biogenic Mn oxide formation. The Mn(II) oxidase was partially purified and found to be resistant to many proteases and active even at high concentrations of sodium dodecyl sulfate. Comparative analysis of the genes involved in Mn(II) oxidation from three diverse Bacillus species revealed a complement of conserved Cu-binding regions not present in well-characterized multicopper oxidases. Our results provide the first direct identification of a bacterial enzyme that catalyzes Mn(II) oxidation and suggest that MnxG catalyzes two sequential one-electron oxidations from Mn(II) to Mn(III) and from Mn(III) to Mn(IV), a novel type of reaction for a multicopper oxidase.
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