ArticleJournal of bacteriology2008
Regulation of circadian clock gene expression by phosphorylation states of KaiC in cyanobacteria.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 25 citations in OpenAlex.
- When lowering temperature, the in vivo circadian clock in cyanobacteria follows and surpasses the in vitro protein clock trough the Hopf bifurcation.Scientific reports · 2025Article
- Archaean green-light environments drove the evolution of cyanobacteria's light-harvesting system.Nature ecology & evolution · 2025Article
- The inner workings of an ancient biological clock.Trends in biochemical sciences · 2024Review
- Translating around the clock: Multi-level regulation of post-transcriptional processes by the circadian clock.Cellular signalling · 2021Review
- Involvement of glycogen metabolism in circadian control of UV resistance in cyanobacteria.PLoS genetics · 2020Article
- Damped circadian oscillation in the absence of KaiA in Synechococcus.Nature communications · 2020Article
- Daily expression pattern of protein-encoding genes and small noncoding RNAs in synechocystis sp. strain PCC 6803.Applied and environmental microbiology · 2014Article
- Hypersensitive photic responses and intact genome-wide transcriptional control without the KaiC phosphorylation cycle in the Synechococcus circadian system.Journal of bacteriology · 2014Article
- Article
- Elucidation of the role of clp protease components in circadian rhythm by genetic deletion and overexpression in cyanobacteria.Journal of bacteriology · 2013Article
- Two antagonistic clock-regulated histidine kinases time the activation of circadian gene expression.Molecular cell · 2013Article
- Revealing a two-loop transcriptional feedback mechanism in the cyanobacterial circadian clock.PLoS computational biology · 2013Article
- Circadian transcriptional regulation by the posttranslational oscillator without de novo clock gene expression in Synechococcus.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- The itty-bitty time machine genetics of the cyanobacterial circadian clock.Advances in genetics · 2011Review
- Dynamic PER repression mechanisms in the Drosophila circadian clock: from on-DNA to off-DNA.Genes & development · 2010Article
- Biochemical evidence for a timing mechanism in prochlorococcus.Journal of bacteriology · 2009Article
- A novel allele of kaiA shortens the circadian period and strengthens interaction of oscillator components in the cyanobacterium Synechococcus elongatus PCC 7942.Journal of bacteriology · 2009Article
- Nonparametric entrainment of the in vitro circadian phosphorylation rhythm of cyanobacterial KaiC by temperature cycle.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- How a cyanobacterium tells time.Current opinion in microbiology · 2008Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three clock proteins--KaiA, KaiB, and KaiC--have been identified as essential components of the circadian oscillator in cyanobacteria, and Kai-based chemical oscillation is thought to be the basic circadian timing mechanism in Synechococcus elongatus PCC 7942. Transcription and translation of kaiBC in cyanobacterial cells was quantitatively studied to elucidate how these processes are coupled to the chemical oscillator using a strain in which circadian oscillation is under the control of IPTG (isopropyl-beta-D-thiogalactopyranoside). The kinetics of repression of kaiBC promoter triggered by IPTG allowed estimation of transient response at 10 h. This response time is suitable for cyanobacterial transcription and/or translation to match with the Kai-based oscillator. Interestingly, kaiBC promoter activity and KaiC phosphorylation showed robust circadian rhythms, whereas trc promoter-driven kaiBC mRNA levels and KaiC accumulation were almost arrhythmic. These results indicate that cyanobacterial circadian rhythms can be generated even if kaiBC expression is constitutive. Moreover, there was a positive correlation between activation of the kaiBC promoter and an increase in the KaiC phosphorylation ratio in three rhythmic conditions. Based on these observations, it is likely that the KaiC phosphorylation ratio is the main factor in the activation of kaiBC promoter. Finally, we quantitatively compared the threshold level of phosphorylated KaiC for the repression or derepression of kaiBC promoter and found that this parameter is an important factor in repressing the kaiBC promoter.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.