ArticleThe Journal of biological chemistry2008
Allosteric activation of the ATPase activity of the Escherichia coli RhlB RNA helicase.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- RNase E resolves toxic condensates by counteracting phase separation of Type II RhlB helicases.Nucleic acids research · 2026Article
- Critical functions and key interactions mediated by the RNase E scaffolding domain in Pseudomonas aeruginosa.PLoS genetics · 2025Article
- Allosteric activation of RhlB by RNase E induces partial duplex opening in substrate RNA.Frontiers in molecular biosciences · 2023Article
- The RNA degradosome promotes tRNA quality control through clearance of hypomodified tRNA.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Poly(A) polymerase is required for RyhB sRNA stability and function inRNA (New York, N.Y.) · 2018Article
- RNase E in the γ-Proteobacteria: conservation of intrinsically disordered noncatalytic region and molecular evolution of microdomains.Molecular genetics and genomics : MGG · 2015Article
- Membrane recognition and dynamics of the RNA degradosome.PLoS genetics · 2015Article
- Potential regulatory interactions of Escherichia coli RraA protein with DEAD-box helicases.The Journal of biological chemistry · 2013Article
- Recognition of the 70S ribosome and polysome by the RNA degradosome in Escherichia coli.Nucleic acids research · 2012Article
- Ribozyme stability, exon skipping, and a potential role for RNA helicase in group I intron splicing by Coxiella burnetii.Journal of bacteriology · 2011Article
- Review
- Characterization of the RNA degradosome of Pseudoalteromonas haloplanktis: conservation of the RNase E-RhlB interaction in the gammaproteobacteria.Journal of bacteriology · 2010Article
- The regulatory protein RraA modulates RNA-binding and helicase activities of the E. coli RNA degradosome.RNA (New York, N.Y.) · 2010Article
- The Q motif of a viral packaging motor governs its force generation and communicates ATP recognition to DNA interaction.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Reconstitution and analysis of the multienzyme Escherichia coli RNA degradosome.Journal of molecular biology · 2008Article
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
Abstract
Helicase B (RhlB) is one of the five DEAD box RNA-dependent ATPases found in Escherichia coli. Unique among these enzymes, RhlB requires an interaction with the partner protein RNase E for appreciable ATPase and RNA unwinding activities. To explore the basis for this activating effect, we have generated a di-cistronic vector that overexpresses a complex comprising RhlB and its recognition site within RNase E, corresponding to residues 696-762. Complex formation has been characterized by isothermal titration calorimetry, revealing an avid, enthalpy-favored interaction between the helicase and RNase E-(696-762) with an equilibrium binding constant (Ka) of at least 1 x 10(8) m(-1). We studied ATPase activity of mutants with substitutions within the ATP binding pocket of RhlB and on the putative interaction surface that mediates recognition of RNase E. For comparisons, corresponding mutations were prepared in two other E. coli DEAD box ATPases, RhlE and SrmB. Strikingly, substitutions at a phenylalanine near the Q-motif found in DEAD box proteins boosts the ATPase activity of RhlB in the absence of RNA, but completely inhibits it in its presence. The data support the proposal that the protein-protein and RNA-binding surfaces both communicate allosterically with the ATPase catalytic center. We conjecture that this communication may govern the mechanical power and efficiency of the helicases, and is tuned in individual helicases in accordance with cellular function.
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