ArticleHepatology (Baltimore, Md.)2008
Microtubule acetylation and stability may explain alcohol-induced alterations in hepatic protein trafficking.
Article in Hepatology (Baltimore, Md.), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 39 citations in OpenAlex.
- Imaging of Microtubules in a Nonhuman Primate Model of Alcohol Use Disorder.ACS pharmacology & translational science · 2025Article
- Micromechanical Study of Hyperacetylated Nucleosomes Using Single Molecule Transverse Magnetic Tweezers.International journal of molecular sciences · 2023Article
- Review
- Alcohol: basic and translational research; 15th annual Charles Lieber &1st Samuel French satellite symposium.Experimental and molecular pathology · 2022Review
- Alcoholic-Hepatitis, Links to Brain and Microbiome: Mechanisms, Clinical and Experimental Research.Biomedicines · 2020Review
- Alcohol-induced microtubule acetylation leads to the accumulation of large, immobile lipid droplets.American journal of physiology. Gastrointestinal and liver physiology · 2019Article
- The cell biology of the hepatocyte: A membrane trafficking machine.The Journal of cell biology · 2019Review
- Rab17 regulates apical delivery of hepatic transcytotic vesicles.Molecular biology of the cell · 2018Article
- Comparative and network-based proteomic analysis of low dose ethanol- and lipopolysaccharide-induced macrophages.PloS one · 2018Article
- Ethanol metabolism by alcohol dehydrogenase or cytochrome PAmerican journal of physiology. Gastrointestinal and liver physiology · 2017Article
- Linking Pathogenic Mechanisms of Alcoholic Liver Disease With Clinical Phenotypes.Gastroenterology · 2016Review
- The Altered Hepatic Tubulin Code in Alcoholic Liver Disease.Biomolecules · 2015Review
- Cholesterol-mediated activation of acid sphingomyelinase disrupts autophagy in the retinal pigment epithelium.Molecular biology of the cell · 2015Article
- Alcohol-induced defects in hepatic transcytosis may be explained by impaired dynein function.Molecular and cellular biochemistry · 2014Article
- Ethanol-induced oxidant stress modulates hepatic autophagy and proteasome activity.Redox biology · 2014Review
- Hepatic microtubule acetylation and stability induced by chronic alcohol exposure impair nuclear translocation of STAT3 and STAT5B, but not Smad2/3.American journal of physiology. Gastrointestinal and liver physiology · 2012Article
- Lysine acetylation induced by chronic ethanol consumption impairs dynamin-mediated clathrin-coated vesicle release.Hepatology (Baltimore, Md.) · 2012Article
- Highly dynamic microtubules improve the effectiveness of early stages of human influenza A/NWS/33 virus infection in LLC-MK2 cells.PloS one · 2012Article
- Lipid droplet accumulation and impaired fat efflux in polarized hepatic cells: consequences of ethanol metabolism.International journal of hepatology · 2012Article
- Alcohol consumption impairs hepatic protein trafficking: mechanisms and consequences.Genes & nutrition · 2010Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
unlabelledWe have been using polarized hepatic WIF-B cells to examine ethanol-induced liver injury. Previously, we determined microtubules were more highly acetylated and more stable in ethanol-treated WIF-B cells. We proposed that the ethanol-induced alterations in microtubule dynamics may explain the ethanol-induced defects in membrane trafficking that have been previously documented. To test this, we compared the trafficking of selected proteins in control cells and cells treated with ethanol or with the histone deacetylase 6 inhibitor trichostatin A (TSA). We determined that exposure to 50 nM TSA for 30 minutes induced microtubule acetylation ( approximately 3-fold increase) and stability to the same extent as did ethanol. As shown previously in situ, the endocytic trafficking of the asialoglycoprotein receptor (ASGP-R) was impaired in ethanol-treated WIF-B cells. This impairment required ethanol metabolism and was likely mediated by acetaldehyde. TSA also impaired ASGP-R endocytic trafficking, but to a lesser extent. Similarly, both ethanol and TSA impaired transcytosis of the single-spanning apical resident aminopeptidase N (APN). For both ASGP-R and APN and for both treatments, the block in trafficking was internalization from the basolateral membrane. Interestingly, no changes in transcytosis of the glycophosphatidylinositol-anchored protein, 5'-nucleotidase, were observed, suggesting that increased microtubule acetylation and stability differentially regulate internalization. We further determined that albumin secretion was impaired in both ethanol-treated and TSA-treated cells, indicating that increased microtubule acetylation and stability also disrupted this transport step.
conclusionThese results indicate that altered microtubule dynamics explain in part alcohol-induced defects in membrane trafficking.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.