ArticleMolecular and cellular biology2008
Centrin/Cdc31 is a novel regulator of protein degradation.
Article in Molecular and cellular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Spatiotemporal control of cortical centrin patterning by regionalized Sfi1 family scaffolding proteins in Stentor coeruleus.Current biology : CB · 2026Article
- Phosphorylation promotes the endonuclease-like activity of human centrin 2.RSC advances · 2022Article
- Structural Basis for the Functional Diversity of Centrins: A Focus on Calcium Sensing Properties and Target Recognition.International journal of molecular sciences · 2021Review
- The role of gene dosage in budding yeast centrosome scaling and spontaneous diploidization.PLoS genetics · 2020Article
- Formation and Recognition of UV-Induced DNA Damage within Genome Complexity.International journal of molecular sciences · 2020Review
- Trypanosoma brucei centrin5 is enriched in the flagellum and interacts with other centrins in a calcium-dependent manner.FEBS open bio · 2019Article
- Article
- Catalytically Active Proteasomes Function Predominantly in the Cytosol.The Journal of biological chemistry · 2016Article
- Rad23 interaction with the proteasome is regulated by phosphorylation of its ubiquitin-like (UbL) domain.Journal of molecular biology · 2014Article
- Regulation of spindle pole body assembly and cytokinesis by the centrin-binding protein Sfi1 in fission yeast.Molecular biology of the cell · 2014Article
- Sfr13, a member of a large family of asymmetrically localized Sfi1-repeat proteins, is important for basal body separation and stability in Tetrahymena thermophila.Journal of cell science · 2013Article
- Structural and functional evidence that Rad4 competes with Rad2 for binding to the Tfb1 subunit of TFIIH in NER.Nucleic acids research · 2013Article
- Such small hands: the roles of centrins/caltractins in the centriole and in genome maintenance.Cellular and molecular life sciences : CMLS · 2012Review
- Review
- Centrin depletion causes cyst formation and other ciliopathy-related phenotypes in zebrafish.Cell cycle (Georgetown, Tex.) · 2011Article
- Defective nucleotide excision repair with normal centrosome structures and functions in the absence of all vertebrate centrins.The Journal of cell biology · 2011Article
- Coordination of centrosome homeostasis and DNA repair is intact in MCF-7 and disrupted in MDA-MB 231 breast cancer cells.Cancer research · 2010Article
- Dictyostelium centrin B localization during cell cycle progression.Communicative & integrative biology · 2010Article
- Rad4 regulates protein turnover at a postubiquitylation step.Molecular biology of the cell · 2010Article
- Dictyostelium discoideum CenB is a bona fide centrin essential for nuclear architecture and centrosome stability.Eukaryotic cell · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Rad23 is required for efficient protein degradation and performs an important role in nucleotide excision repair. Saccharomyces cerevisiae Rad23, and its human counterpart (hHR23), are present in a complex containing the DNA repair factor Rad4 (termed XPC, for xeroderma pigmentosum group C, in humans). XPC/hHR23 was also reported to bind centrin-2, a member of the superfamily of calcium-binding EF-hand proteins. We report here that yeast centrin, which is encoded by CDC31, is similarly present in a complex with Rad4/Rad23 (called NEF2). The interaction between Cdc31 and Rad23/Rad4 varied by growth phase and reflected oscillations in Cdc31 levels. Strikingly, a cdc31 mutant that formed a weaker interaction with Rad4 showed sensitivity to UV light. Based on the dual function of Rad23, in both DNA repair and protein degradation, we questioned if Cdc31 also participated in protein degradation. We report here that Cdc31 binds the proteasome and multiubiquitinated proteins through its carboxy-terminal EF-hand motifs. Moreover, cdc31 mutants were highly sensitive to drugs that cause protein damage, failed to efficiently degrade proteolytic substrates, and formed altered interactions with the proteasome. These findings reveal for the first time a new role for centrin/Cdc31 in protein degradation.
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Registered trials
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