ArticleProceedings of the National Academy of Sciences of the United States of America2008
Three-dimensional cellular microarray for high-throughput toxicology assays.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 104 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
104 citing papers in PubMed.
- Cell-embedded microgels as emerging miniature 3D tissue-mimics toward biochip-based toxicity screening.Bioengineering & translational medicine · 2026Review
- Development of Sheep Duodenum Intestinal Organoids and Implementation of High-Throughput Screening Platform for Veterinary Applications.International journal of molecular sciences · 2025Article
- Reproducible generation of human liver organoids (HLOs) on a pillar plate platformLab on a chip · 2024Article
- Advances in high throughput cell culture technologies for therapeutic screening and biological discovery applications.Bioengineering & translational medicine · 2024Review
- Bioinformatics Study of Flavonoids From GenusAdvances and applications in bioinformatics and chemistry : AABC · 2024Article
- Systems approaches to uncovering the contribution of environment-mediated drug resistance.Current opinion in solid state & materials science · 2022Article
- Application of microfluidic chips in anticancer drug screening.Bosnian journal of basic medical sciences · 2022Review
- Review
- Microcrater-Arrayed Chemiluminescence Cell Chip to Boost Anti-Cancer Drug Administration in Zebrafish Tumor Xenograft Model.Biology · 2021Article
- Analysis of Static Molecular Gradients in a High-Throughput Drug Screening Microfluidic Assay.Molecules (Basel, Switzerland) · 2021Article
- Fabrication approaches for high-throughput and biomimetic disease modeling.Acta biomaterialia · 2021Review
- Recent advances in microarray 3D bioprinting for high-throughput spheroid and tissue culture and analysis.Essays in biochemistry · 2021Review
- Alginate Microencapsulation for Three-Dimensional In Vitro Cell Culture.ACS biomaterials science & engineering · 2021Review
- [Research advances of high-throughput cell-based drug screening systems based on microfluidic technique].Se pu = Chinese journal of chromatography · 2021Review
- High-Throughput Screening of Compound Neurotoxicity Using 3D-Cultured Neural Stem Cells on a 384-Pillar Plate.Current protocols · 2021Article
- The Alginate Immobilization of Metabolic Enzymes Platform Retrofits an Estrogen Receptor Transactivation Assay With Metabolic Competence.Toxicological sciences : an official journal of the Society of Toxicology · 2020Article
- High-throughput multiplex assays with mouse macrophages on pillar plate platforms.Experimental cell research · 2020Article
- Micropillar/Microwell Chip Assessment for Detoxification of Bisphenol A with Korean Pear (Micromachines · 2020Article
- Astrocytes Decreased the Sensitivity of Glioblastoma Cells to Temozolomide and Bay 11-7082.International journal of molecular sciences · 2020Article
- Integrating Biophysics in Toxicology.Cells · 2020Review
44 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
We have developed a miniaturized 3D cell-culture array (the Data Analysis Toxicology Assay Chip or DataChip) for high-throughput toxicity screening of drug candidates and their cytochrome P450-generated metabolites. The DataChip consists of human cells encapsulated in collagen or alginate gels (as small as 20 nl) arrayed on a functionalized glass slide for spatially addressable screening against multiple compounds. A single DataChip containing 1,080 individual cell cultures, used in conjunction with the complementary human P450-containing microarray (the Metabolizing Enzyme Toxicology Assay Chip or MetaChip), simultaneously provided IC(50) values for nine compounds and their metabolites from CYP1A2, CYP2D6, and CYP3A4 and a mixture of the three P450s designed to emulate the human liver. Similar responses were obtained with the DataChip and conventional 96-well plate assays, demonstrating that the near 2,000-fold miniaturization does not influence the cytotoxicity response. The DataChip may therefore enable toxicity analyses of drug candidates and their metabolites at throughputs compatible with the availability of compounds at early-stage drug discovery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.