ArticleJournal of clinical microbiology2008
DNA microarray based on arrayed-primer extension technique for identification of pathogenic fungi responsible for invasive and superficial mycoses.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Omics and Multiomics-Based Diagnostics for Invasive Candidiasis: Toward Precision Medicine.Molecular & cellular proteomics : MCP · 2025Review
- Dermatophytosis in context of Trichophyton rubrum: host defense mechanisms, virulence factors, and treatment innovations.Molecular biology reports · 2025Review
- Fluorescent toys 'n' tools lighting the way in fungal research.FEMS microbiology reviews · 2021Article
- Visible DNA microarray and loop-mediated isothermal amplification (LAMP) for the identification of Cryptococcus species recovered from culture medium and cerebrospinal fluid of patients with meningitis.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2020Article
- The Composition of Fungal Communities in the Rumen of Gayals (Polish journal of microbiology · 2019Article
- Identification of nine cryptic species of Candida albicans, C. glabrata, and C. parapsilosis complexes using one-step multiplex PCR.BMC infectious diseases · 2018Article
- Article
- Advances in Candida detection platforms for clinical and point-of-care applications.Critical reviews in biotechnology · 2017Review
- Evaluation of Fluorescent Capillary Electrophoresis for Rapid Identification of Candida Fungal Infections.Journal of clinical microbiology · 2016Article
- Solid and Suspension Microarrays for Microbial Diagnostics.Methods in microbiology · 2015Article
- Visual analysis of DNA microarray data for accurate molecular identification of non-albicans Candida isolates from patients with candidemia episodes.Journal of clinical microbiology · 2013Article
- Accurate and rapid identification of Candida spp. frequently associated with fungemia by using PCR and the microarray-based Prove-it Sepsis assay.Journal of clinical microbiology · 2012Article
- Diagnosis of invasive fungal infections by a real-time panfungal PCR assay in immunocompromised pediatric patients.Leukemia · 2010Article
- Identification of fungal species by fragment length analysis of the internally transcribed spacer 2 region.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2009Article
- Development and validation of a microsphere-based Luminex assay for rapid identification of clinically relevant aspergilli.Journal of clinical microbiology · 2009Article
- Species-specific identification of a wide range of clinically relevant fungal pathogens by use of Luminex xMAP technology.Journal of clinical microbiology · 2009Article
- Colony multiplex-tandem PCR for rapid, accurate identification of fungal cultures.Journal of clinical microbiology · 2008Article
- Diagnosis ofCurrent fungal infection reportsReview
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An oligonucleotide microarray based on the arrayed-primer extension (APEX) technique has been developed to simultaneously identify pathogenic fungi frequently isolated from invasive and superficial infections. Species-specific oligonucleotide probes complementary to the internal transcribed spacer 1 and 2 (ITS1 and ITS2) region were designed for 24 species belonging to 10 genera, including Candida species (Candida albicans, Candida dubliniensis, Candida famata, Candida glabrata, Candida tropicalis, Candida kefyr, Candida krusei, Candida guilliermondii, Candida lusitaniae, Candida metapsilosis, Candida orthopsilosis, Candida parapsilosis, and Candida pulcherrima), Cryptococcus neoformans, Aspergillus species (Aspergillus fumigatus and Aspergillus terreus), Trichophyton species (Trichophyton rubrum and Trichophyton tonsurans), Trichosporon cutaneum, Epidermophyton floccosum, Fusarium solani, Microsporum canis, Penicillium marneffei, and Saccharomyces cerevisiae. The microarray was tested for its specificity with a panel of reference and blinded clinical isolates. The APEX technique was proven to be highly discriminative, leading to unequivocal identification of each species, including the highly related ones C. parapsilosis, C. orthopsilosis, and C. metapsilosis. Because of the satisfactory basic performance traits obtained, such as reproducibility, specificity, and unambiguous interpretation of the results, this new system represents a reliable method of potential use in clinical laboratories for parallel one-shot detection and identification of the most common pathogenic fungi.
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