ArticleJournal of virology2008
cis- and trans-acting functions of brome mosaic virus protein 1a in genomic RNA1 replication.
Article in Journal of virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Review
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- Within-host Evolution of Segments Ratio for the Tripartite Genome of Alfalfa Mosaic Virus.Scientific reports · 2017Article
- The Barley stripe mosaic virus γb protein promotes chloroplast-targeted replication by enhancing unwinding of RNA duplexes.PLoS pathogens · 2017Article
- A self-perpetuating repressive state of a viral replication protein blocks superinfection by the same virus.PLoS pathogens · 2017Article
- Mutational analysis of hepatitis E virus ORF1 "Y-domain": Effects on RNA replication and virion infectivity.World journal of gastroenterology · 2017Article
- Phosphorylation of the Brome Mosaic Virus Capsid Regulates the Timing of Viral Infection.Journal of virology · 2016Article
- Sequence analysis reveals a conserved extension in the capping enzyme of the alphavirus supergroup, and a homologous domain in nodaviruses.Biology direct · 2015Article
- Hepatitis C virus RNA replication depends on specific cis- and trans-acting activities of viral nonstructural proteins.PLoS pathogens · 2015Article
- The bZIP transcription factor HY5 interacts with the promoter of the monoterpene synthase gene QH6 in modulating its rhythmic expression.Frontiers in plant science · 2015Article
- Replication protein of tobacco mosaic virus cotranslationally binds the 5' untranslated region of genomic RNA to enable viral replication.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The bean pod mottle virus RNA2-encoded 58-kilodalton protein P58 is required in cis for RNA2 accumulation.Journal of virology · 2014Article
- Molecular biology and epidemiology of dianthoviruses.Advances in virus research · 2013Review
- The coat protein leads the way: an update on basic and applied studies with the Brome mosaic virus coat protein.Molecular plant pathology · 2011Review
- HSP70 and its cochaperone CPIP promote potyvirus infection in Nicotiana benthamiana by regulating viral coat protein functions.The Plant cell · 2010Article
- The 3' end of Turnip crinkle virus contains a highly interactive structure including a translational enhancer that is disrupted by binding to the RNA-dependent RNA polymerase.RNA (New York, N.Y.) · 2009Article
- Turnip mosaic virus RNA replication complex vesicles are mobile, align with microfilaments, and are each derived from a single viral genome.Journal of virology · 2009Article
- RNA binding by the brome mosaic virus capsid protein and the regulation of viral RNA accumulation.Journal of molecular biology · 2009Article
- Brome mosaic virus capsid protein regulates accumulation of viral replication proteins by binding to the replicase assembly RNA element.RNA (New York, N.Y.) · 2009Article
- Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA viruses employ a combination of mechanisms to regulate their gene expression and replication. Brome mosaic virus (BMV) is a tripartite positive-strand RNA virus used to study the requirements for virus infection. BMV genomic RNA1 encodes protein 1a, which contains a methyltransferase (MT) domain and a helicase domain that are required for replication. 1a forms a complex with the 2a RNA-dependent RNA polymerase for the replication and transcription of all BMV RNAs. RNA1 expressed with 2a from Agrobacterium-based vectors can result in RNA1 replication in Nicotiana benthamiana. A mutation in the 1a translation initiation codon significantly decreased RNA1 accumulation even when wild-type (WT) 1a and 2a were provided in trans. Therefore, efficient RNA1 replication requires 1a translation from RNA1 in cis, indicating a linkage between replication and translation. Mutation analyses showed that the full-length 1a protein was required for efficient RNA1 replication, not just the process of translation. Three RNA1s with mutations in the 1a MT domain could be partially rescued by WT 1a expressed in trans, indicating that the cis-acting function of 1a was retained. Furthermore, an RNA motif in the 5'-untranslated region of RNA1, named the B box, was required for 1a to function in cis and in trans for BMV RNA accumulation. The B box is required for the formation of the replication factory (M. Schwartz, J. Chen, M. Janda, M. Sullivan, J. den Boon, and P. Ahlquist, Mol. Cell 9:505-514, 2002). Results in this work demonstrate a linkage between BMV RNA1 translation and replication.
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