Evidence map›Paper›PMID 18160131›Full record

ArticleJournal of biochemical and biophysical methods2008

Simultaneous measurements of fast optical and proton current kinetics in the bacteriorhodopsin photocycle using an enhanced spectrophotometer.

John W Kakareka, Paul D Smith, Thomas J Pohida, Richard W Hendler

Open access · greenAbstract read
In one paragraph

Article in Journal of biochemical and biophysical methods, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 92% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

John W KakarekaDivision of Computational Bioscience, Center for Information Technology, Bethesda, Maryland, 20892, United States.
Paul D Smith
Thomas J Pohida
Richard W Hendler
Center for Information Technology · USNational Heart Lung and Blood Institute · USNational Institute of Biomedical Imaging and Bioengineering · US

Funding

Signal Processing And Instrumentation SectionZIACT000261 · CIT · CENTER FOR INFORMATION TECHNOLOGY · PI POHIDA, THOMAS J · 2009 to 2021
$16.7M
Instrumentation and Bioengineering Development and ApplicationZIAEB000011 · NIBIB · NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING · PI SMITH, PAUL · 2009 to 2013
$1.4M
Mechanism of energy transduction by bacteriorhodopsinZ01HL000518 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI HENDLER, RICHARD W · 2004 to 2008
$1.0M
Intramural NIH HHS Z01 HL000518Intramural NIH HHS Z99 HL999999
6 · The paper itself

Abstract

A one-of-a-kind high speed optical multichannel spectrometer was designed and built at NIH and described in this journal in 1997 [J.W. Cole, R.W. Hendler, P.D. Smith, H.A. Fredrickson, T.J. Pohida, W.S. Friauf. A high speed optical multichannel analyzer. J Biochem Biophys Methods 1997;35:16-174.]. The most unique aspect of this instrument was the ability to follow an entire time course from a single activation using a single sample. The instrument has been used to study rapid kinetic processes in the photon-driven bacteriorhodopsin photocycle and electron transport from cytochrome c to cytochrome aa3 and from cytochrome aa3 to oxygen. The present paper describes a second generation instrument with a number of important enhancements which significantly improve its capabilities for multichannel kinetic studies. An example application is presented in which the kinetics of photon-induced proton flow across the biological membrane is measured simultaneously with the individual steps of the photocycle determined optically. Matching the time constants for the two processes indicates which molecular transformations are associated with major proton movements.

Indexed as

ProtonsBacteriorhodopsinsElectronsHalobacterium salinarumKineticsPhotochemistrySpectrophotometryTime FactorsBacteriorhodopsinsProtons

Identifiers

PMID18160131
PMCPMC2836842
OpenAlexW1990213876

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.