ArticleBMC microbiology2007
Characterization of the Trichomonas vaginalis surface-associated AP65 and binding domain interacting with trichomonads and host cells.
Article in BMC microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 50 citations in OpenAlex.
- Evaluation of the effect of metronidazole administration on the pathophysiology of trichomonas vaginalis infection among pregnant women in Ghana.BMC infectious diseases · 2026Article
- Recent Advances in Biology, Host and Microbe Interactions of the Human Sexually Transmitted ParasiteInternational journal of molecular sciences · 2025Review
- Trichomonas vaginalis adhesion protein 65 facilitates human papillomavirus entry via SPCS1-mediated upregulation of CD151 and HSPG2 in keratinocyte lineage.Infectious diseases of poverty · 2025Article
- Metronidazole Activation by a Deeply Entangled Dimeric Malic Enzyme inPathogens (Basel, Switzerland) · 2025Article
- Commensal protist Tritrichomonas musculus exhibits a dynamic life cycle that induces extensive remodeling of the gut microbiota.The ISME journal · 2024Article
- Microscopic and molecular detection ofFrontiers in microbiology · 2024Article
- Aptamer-Based Technologies for Parasite Detection.Sensors (Basel, Switzerland) · 2023Review
- Development of a convenient detection method for Trichomonas vaginalis based on loop-mediated isothermal amplification targeting adhesion protein 65.BMC infectious diseases · 2020Article
- The Molecular Characterization and Immunity Identification ofFrontiers in microbiology · 2020Article
- A Cell Surface Aggregation-Promoting Factor from Lactobacillus gasseri Contributes to Inhibition of Trichomonas vaginalis Adhesion to Human Vaginal Ectocervical Cells.Infection and immunity · 2018Article
- A secreted Heat shock protein 90 of Trichomonas vaginalis.PLoS neglected tropical diseases · 2018Article
- Structural basis of interaction between dimeric cyclophilin 1 and Myb1 transcription factor in Trichomonas vaginalis.Scientific reports · 2018Article
- Differential Protein Expressions in Virus-Infected and UninfectedThe Korean journal of parasitology · 2017Article
- The Glycolytic Enzyme Triosephosphate Isomerase of Trichomonas vaginalis Is a Surface-Associated Protein Induced by Glucose That Functions as a Laminin- and Fibronectin-Binding Protein.Infection and immunity · 2016Article
- Trichomoniasis - are we giving the deserved attention to the most common non-viral sexually transmitted disease worldwide?Microbial cell (Graz, Austria) · 2016Review
- The Pathogenesis of Human Cervical Epithelium Cells Induced by Interacting with Trichomonas vaginalis.PloS one · 2015Article
- Effect of recombinant prophenin 2 on the integrity and viability of Trichomonas vaginalis.BioMed research international · 2015Article
- Regulation of nuclear translocation of the Myb1 transcription factor by TvCyclophilin 1 in the protozoan parasite Trichomonas vaginalis.The Journal of biological chemistry · 2014Article
- Epitopes of the highly immunogenic Trichomonas vaginalis α-actinin are serodiagnostic targets for both women and men.Journal of clinical microbiology · 2013Article
- Identification, characterization, and synthesis of peptide epitopes and a recombinant six-epitope protein for Trichomonas vaginalis serodiagnosis.ImmunoTargets and therapy · 2013Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundAP65 is a prominent adhesin of Trichomonas vaginalis that mediates binding of parasites to host vaginal epithelial cells (VECs). AP65 with no secretion signal sequence, membrane targeting peptide, and anchoring motif was recently found to be secreted.
resultsWe first wanted to demonstrate surface association of AP65 to the parasite followed by the identification of the binding epitope interacting with both organisms and VECs. AP65 was found to bind to trichomonads, but not to trypsin-treated parasites, in an auto-ligand assay, suggesting the existence of a surface protein associating with AP65. Since rabbit antiserum IgG antibodies reactive with epitopes localized to the N-terminal region of AP65 inhibit the attachment of live parasites to VECs, we hypothesized that the binding domain was localized to this region. We subcloned five overlapping fragments of AP65 called c1 through c5, and expression of recombinant clones was confirmed with antibodies to AP65. Each purified recombinant protein was then tested for binding activity using an established ligand assay, and fragment c1 with the first twenty-five amino acids in the N-terminal domain was required for binding to VECs and, surprisingly, also to parasites. Importantly, c1 competed with the binding of AP65 to both cells types.
conclusionT. vaginalis AP65 is a secreted, surface-associated protein and a model is proposed to explain how this secreted protein functions as an adhesin.
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