ArticleProtoplasma2007
Relationships between transcription, silver staining, and chromatin organization of nucleolar organizers in Secale cereale.
Article in Protoplasma, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Does the chromosomal position of 35S rDNA sites influence their transcription? A survey on Nothoscordum species (Amaryllidaceae).Genetics and molecular biology · 2020Article
- Karyotype structure and NOR activity in BrazilianComparative cytogenetics · 2019Article
- Functional ultrastructure of the plant nucleolus.Protoplasma · 2014Review
- Article
- Chemical probes in plant epigenetics studies.Plant signaling & behavior · 2013Review
- Cytogenetic analysis of B chromosomes in one population of the fish Moenkhausia sanctaefilomenae (Steindachner, 1907) (Teleostei, Characiformes).Comparative cytogenetics · 2012Article
- Telomere and 45S rDNA sequences are structurally linked on the chromosomes in Chrysanthemum segetum L.Protoplasma · 2012Article
- Identification of DHX33 as a mediator of rRNA synthesis and cell growth.Molecular and cellular biology · 2011Article
- MeCP2 is required for global heterochromatic and nucleolar changes during activity-dependent neuronal maturation.Neurobiology of disease · 2011Article
- Article
- Nucleolar dominance and ribosomal RNA gene silencing.Current opinion in cell biology · 2010Article
- Visualization by atomic force microscopy and FISH of the 45S rDNA gaps in mitotic chromosomes of Lolium perenne.Protoplasma · 2009Article
- Quantitative analysis of NOR expression in a B chromosome of the grasshopper Eyprepocnemis plorans.Chromosoma · 2009Article
- Interplay of ribosomal DNA loci in nucleolar dominance: dominant NORs are up-regulated by chromatin dynamics in the wheat-rye system.PloS one · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The nucleolar organizer regions (NORs) are composed of hundreds of rRNA genes, typically spanning several megabases. Cytologically, NORs include regions that are highly condensed and regions that are decondensed, the latter corresponding to regions at which associated proteins stain intensively with silver (Ag-NORs) and where active rRNA gene transcription is thought to occur. To test the relationship between rRNA gene activity, NOR silver staining, and rDNA (genes coding for rRNA) chromatin condensation, we used the DNA methyl-transferase inhibitor 5-azacytidine to evaluate the correlation between the epigenetic regulation of rRNA genes and NOR silver staining in the plant Secale cereale. Following 5-azacytidine treatment, we observed an increase in rRNA gene transcription as well as a reduction in the number of cells showing a significant difference in the size of the silver-stained domains in the two NORs. These transcriptional changes occurred concomitantly with an increase in nuclear and nucleolar size and were associated with the reallocation of most of the rDNA from perinucleolar heterochromatin into the nucleolus. Collectively, these results suggest that rRNA gene transcription, silver staining, and NOR decondensation are interrelated in S. cereale.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.