ArticleBritish journal of pharmacology2008
Skeletal muscle expresses the extracellular cyclic AMP-adenosine pathway.
Article in British journal of pharmacology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 62 citations in OpenAlex.
- Extracellular cAMP-Adenosine Pathway Signaling: A Potential Therapeutic Target in Chronic Inflammatory Airway Diseases.Frontiers in immunology · 2022Review
- A novel secreted-cAMP pathway inhibits pulmonary hypertension via a feed-forward mechanism.Cardiovascular research · 2020Article
- Article
- Harnessing cAMP signaling in musculoskeletal regenerative engineering.Drug discovery today · 2017Review
- Myobolites: muscle-derived metabolites with paracrine and systemic effects.Current opinion in pharmacology · 2017Review
- Placental ABC Transporters: Biological Impact and Pharmaceutical Significance.Pharmaceutical research · 2016Review
- Purines: forgotten mediators in traumatic brain injury.Journal of neurochemistry · 2016Review
- Article
- New perspectives in signaling mediated by receptors coupled to stimulatory G protein: the emerging significance of cAMP efflux and extracellular cAMP-adenosine pathway.Frontiers in pharmacology · 2015Review
- Cardiac myocyte-secreted cAMP exerts paracrine action via adenosine receptor activation.The Journal of clinical investigation · 2014Article
- Allosteric interactions at adenosine A(1) and A(3) receptors: new insights into the role of small molecules and receptor dimerization.British journal of pharmacology · 2014Review
- Article
- Cellular function and molecular structure of ecto-nucleotidases.Purinergic signalling · 2012Review
- The 2',3'-cAMP-adenosine pathway.American journal of physiology. Renal physiology · 2011Review
- Expression of the 2',3'-cAMP-adenosine pathway in astrocytes and microglia.Journal of neurochemistry · 2011Article
- Extracellular cAMP-adenosine pathways in the mouse kidney.American journal of physiology. Renal physiology · 2011Article
- 2',3'-cAMP, 3'-AMP, and 2'-AMP inhibit human aortic and coronary vascular smooth muscle cell proliferation via A2B receptors.American journal of physiology. Heart and circulatory physiology · 2011Article
- Endogenous luminal surface adenosine signaling regulates duodenal bicarbonate secretion in rats.The Journal of pharmacology and experimental therapeutics · 2010Article
- Extracellular 3',5'-cAMP-adenosine pathway inhibits glomerular mesangial cell growth.The Journal of pharmacology and experimental therapeutics · 2010Article
- Multidrug resistance protein 4 mediates cAMP efflux from rat preglomerular vascular smooth muscle cells.Clinical and experimental pharmacology & physiology · 2010Article
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background and purposecAMP is a key intracellular signalling molecule that regulates multiple processes of the vertebrate skeletal muscle. We have shown that cAMP can be actively pumped out from the skeletal muscle cell. Since in other tissues, cAMP efflux had been associated with extracellular generation of adenosine, in the present study we have assessed the fate of interstitial cAMP and the existence of an extracellular cAMP-adenosine signalling pathway in skeletal muscle. EXPERIMENTAL APPROACH: cAMP efflux and/or its extracellular degradation were analysed by incubating rat cultured skeletal muscle with exogenous cAMP, forskolin or isoprenaline. cAMP and its metabolites were quantified by radioassay or HPLC, respectively. KEY
resultsIncubation of cells with exogenous cAMP was followed by interstitial accumulation of 5'-AMP and adenosine, a phenomenon inhibited by selective inhibitors of ecto-phosphodiesterase (DPSPX) and ecto-nucleotidase (AMPCP). Activation of adenylyl cyclase (AC) in cultured cells with forskolin or isoprenaline increased cAMP efflux and extracellular generation of 5'-AMP and adenosine. Extracellular cAMP-adenosine pathway was also observed after direct and receptor-dependent stimulation of AC in rat extensor muscle ex vivo. These events were attenuated by probenecid, an inhibitor of ATP binding cassette family transporters. CONCLUSIONS AND IMPLICATIONS: Our results show the existence of an extracellular biochemical cascade that converts cAMP into adenosine. The functional relevance of this extracellular signalling system may involve a feedback modulation of cellular response initiated by several G protein-coupled receptor ligands, amplifying cAMP influence to a paracrine mode, through its metabolite, adenosine.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.