ArticleProtein science : a publication of the Protein Society2008
Crystal structure of 5-methylthioribose 1-phosphate isomerase product complex from Bacillus subtilis: implications for catalytic mechanism.
Article in Protein science : a publication of the Protein Society, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 26 citations in OpenAlex.
- Methionine Salvage Enzyme Uses a Unique Mechanism to Overcome a Challenging Aldose-Ketose Isomerization.Journal of the American Chemical Society · 2025Article
- Sugar phosphate activation of the stress sensor eIF2B.Nature communications · 2021Article
- The Prodigal Compound: Return of Ribosyl 1,5-Bisphosphate as an Important Player in Metabolism.Microbiology and molecular biology reviews : MMBR · 2019Review
- Revisiting the methionine salvage pathway and its paralogues.Microbial biotechnology · 2019Article
- Architecture of the eIF2B regulatory subcomplex and its implications for the regulation of guanine nucleotide exchange on eIF2.Nucleic acids research · 2015Article
- Insights into the architecture of the eIF2Bα/β/δ regulatory subcomplex.Biochemistry · 2014Article
- Identification of a 5'-deoxyadenosine deaminase in Methanocaldococcus jannaschii and its possible role in recycling the radical S-adenosylmethionine enzyme reaction product 5'-deoxyadenosine.Journal of bacteriology · 2014Article
- Structure of mediator of RhoA-dependent invasion (MRDI) explains its dual function as a metabolic enzyme and a mediator of cell invasion.Biochemistry · 2013Article
- MtnBD is a multifunctional fusion enzyme in the methionine salvage pathway of Tetrahymena thermophila.PloS one · 2013Article
- Enzymatic characterization of AMP phosphorylase and ribose-1,5-bisphosphate isomerase functioning in an archaeal AMP metabolic pathway.Journal of bacteriology · 2012Article
- Dynamic, ligand-dependent conformational change triggers reaction of ribose-1,5-bisphosphate isomerase from Thermococcus kodakarensis KOD1.The Journal of biological chemistry · 2012Article
- A mediator of Rho-dependent invasion moonlights as a methionine salvage enzyme.Molecular & cellular proteomics : MCP · 2009Article
- Structural and functional similarities between a ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO)-like protein from Bacillus subtilis and photosynthetic RuBisCO.The Journal of biological chemistry · 2009Article
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7 authors at 2 institutions in 1 country.
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Abstract
The methionine salvage pathway (MSP) plays a crucial role in recycling a sulphahydryl derivative of the nucleoside. Recently, the genes and reactions in MSP from Bacillus subtilis have been identified, where 5-methylthioribose 1-phosphate isomerase (M1Pi) catalyzes a conversion of 5-methylthioribose 1-phosphate (MTR-1-P) to 5-methylthioribulose 1-phosphate (MTRu-1-P). Herein, we report the crystal structures of B. subtilis M1Pi (Bs-M1Pi) in complex with its product MTRu-1-P, and a sulfate at 2.4 and 2.7 A resolution, respectively. The electron density clearly shows the presence of each compound in the active site. The structural comparison with other homologous proteins explains how the substrate uptake of Bs-M1Pi may be induced by an open/closed transition of the active site. The highly conserved residues at the active site, namely, Cys160 and Asp240 are most likely to be involved in catalysis. The structural analysis sheds light on its catalytic mechanism of M1Pi.
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