ArticlePlant physiology2008
Dual targeting of Arabidopsis holocarboxylase synthetase1: a small upstream open reading frame regulates translation initiation and protein targeting.
Article in Plant physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 48 citations in OpenAlex.
- Overlapping upstream ORFs repress translation and expand proteome diversity in Arabidopsis.bioRxiv : the preprint server for biology · 2026Article
- Fine-tuning of conditional Transformers improvesComputational and structural biotechnology journal · 2025Article
- Evaluation of Eukaryotic mRNA Coding Potential.Methods in molecular biology (Clifton, N.J.) · 2025Article
- What, where, and how: Regulation of translation and the translational landscape in plants.The Plant cell · 2024Review
- Improved super-resolution ribosome profiling reveals prevalent translation of upstream ORFs and small ORFs in Arabidopsis.The Plant cell · 2024Article
- Calmodulin is involved in the dual subcellular location of two chloroplast proteins.The Journal of biological chemistry · 2019Article
- Host biotin is required for liver stage development in malaria parasites.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Genome-wide association mapping and Identification of candidate genes for fatty acid composition in Brassica napus L. using SNP markers.BMC genomics · 2017Article
- Dual-targeting of Arabidopsis DMP1 isoforms to the tonoplast and the plasma membrane.PloS one · 2017Article
- An appropriate concentration of arginine is required for normal root growth in rice.Plant signaling & behavior · 2014Article
- Article
- Translational Regulation of Cytoplasmic mRNAs.The arabidopsis book · 2013Article
- Article
- Reverse-genetic analysis of the two biotin-containing subunit genes of the heteromeric acetyl-coenzyme A carboxylase in Arabidopsis indicates a unidirectional functional redundancy.Plant physiology · 2011Article
- Conserved alternative splicing of Arabidopsis transthyretin-like determines protein localization and S-allantoin synthesis in peroxisomes.The Plant cell · 2010Article
- Conservation of dual-targeted proteins in Arabidopsis and rice points to a similar pattern of gene-family evolution.Molecular genetics and genomics : MGG · 2009Article
- Tyrosine and phenylalanine are synthesized within the plastids in Arabidopsis.Plant physiology · 2009Article
- Participation of leaky ribosome scanning in protein dual targeting by alternative translation initiation in higher plants.The Plant cell · 2009Article
- The Omp85-related chloroplast outer envelope protein OEP80 is essential for viability in Arabidopsis.Plant physiology · 2008Article
- A versatile Escherichia coli strain for identification of biotin transporters and for biotin quantification.BioengineeredArticle
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein biotinylation is an original and very specific posttranslational modification, compartmented in plants, between mitochondria, plastids, and the cytosol. This reaction modifies and activates few carboxylases committed in key metabolisms and is catalyzed by holocarboxylase synthetase (HCS). The molecular bases of this complex compartmentalization and the relative function of each of the HCS genes, HCS1 and HCS2, identified in Arabidopsis (Arabidopsis thaliana) are mainly unknown. Here, we showed by reverse genetics that the HCS1 gene is essential for plant viability, whereas disruption of the HCS2 gene in Arabidopsis does not lead to any obvious phenotype when plants are grown under standard conditions. These findings strongly suggest that HCS1 is the only protein responsible for HCS activity in Arabidopsis cells, including the cytosolic, mitochondrial, and plastidial compartments. A closer study of HCS1 gene expression enabled us to propose an original mechanism to account for this multiplicity of localizations. Located in the HCS1 messenger RNA 5'-untranslated region, an upstream open reading frame regulates the translation initiation of HCS1 and the subsequent targeting of HCS1 protein. Moreover, an exquisitely precise alternative splicing of HCS1 messenger RNA can regulate the presence and absence of this upstream open reading frame. The existence of these complex and interdependent mechanisms creates a rich molecular platform where different parameters and factors could control HCS targeting and hence biotin metabolism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.