ArticleEukaryotic cell2008
Role of heme in the antifungal activity of the azaoxoaporphine alkaloid sampangine.
Article in Eukaryotic cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 46 citations in OpenAlex.
- Prospective on Alkaloids-based sustainable methods to treat fungal pathogens: a comprehensive review.Archives of microbiology · 2025Review
- The Interkingdom Interaction withJournal of microbiology and biotechnology · 2023Article
- Antibacterial and Antifungal Alkaloids from Asian Angiosperms: Distribution, Mechanisms of Action, Structure-Activity, and Clinical Potentials.Antibiotics (Basel, Switzerland) · 2022Review
- Natural products for infectious microbes and diseases: an overview of sources, compounds, and chemical diversities.Science China. Life sciences · 2022Review
- Biological Activity of Naturally Derived Naphthyridines.Molecules (Basel, Switzerland) · 2021Review
- Oxoisoaporphines and Aporphines: Versatile Molecules with Anticancer Effects.Molecules (Basel, Switzerland) · 2019Review
- The Still Underestimated Problem of Fungal Diseases Worldwide.Frontiers in microbiology · 2019Review
- Dermatophyte Resistance to Antifungal Drugs: Mechanisms and Prospectus.Frontiers in microbiology · 2018Review
- Protocol for Identifying Natural Agents That Selectively Affect Adhesion, Thickness, Architecture, Cellular Phenotypes, Extracellular Matrix, and Human White Blood Cell Impenetrability of Candida albicans Biofilms.Antimicrobial agents and chemotherapy · 2017Article
- Novel Cell-Killing Mechanisms of Hydroxyurea and the Implication toward Combination Therapy for the Treatment of Fungal Infections.Antimicrobial agents and chemotherapy · 2017Article
- Two plant-derived aporphinoid alkaloids exert their antifungal activity by disrupting mitochondrial iron-sulfur cluster biosynthesis.The Journal of biological chemistry · 2017Article
- New Horizons in Antifungal Therapy.Journal of fungi (Basel, Switzerland) · 2016Review
- YeastNet v3: a public database of data-specific and integrated functional gene networks for Saccharomyces cerevisiae.Nucleic acids research · 2014Article
- Transcriptional profile of Paracoccidioides induced by oenothein B, a potential antifungal agent from the Brazilian Cerrado plant Eugenia uniflora.BMC microbiology · 2013Article
- A potent plant-derived antifungal acetylenic acid mediates its activity by interfering with fatty acid homeostasis.Antimicrobial agents and chemotherapy · 2012Article
- Sampangine inhibits heme biosynthesis in both yeast and human.Eukaryotic cell · 2011Article
- Azaphenanthrene alkaloids with antitumoral activity from Anaxagorea dolichocarpa Sprague & Sandwith (Annonaceae).Molecules (Basel, Switzerland) · 2011Article
- Natural Product Based 6-Hydroxy-2,3,4,6-tetrahydropyrrolo[1,2-a]pyrimidinium Scaffold as A New Antifungal Template.ACS medicinal chemistry letters · 2011Article
- The marine sponge-derived polyketide endoperoxide plakortide F acid mediates its antifungal activity by interfering with calcium homeostasis.Antimicrobial agents and chemotherapy · 2011Article
- The fungal pathogen Candida albicans autoinduces hyphal morphogenesis by raising extracellular pH.mBio · 2011Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Sampangine, a plant-derived alkaloid found in the Annonaceae family, exhibits strong inhibitory activity against the opportunistic fungal pathogens Candida albicans, Cryptococcus neoformans, and Aspergillus fumigatus. In the present study, transcriptional profiling experiments coupled with analyses of mutants were performed in an effort to elucidate its mechanism of action. Using Saccharomyces cerevisiae as a model organism, we show that sampangine produces a transcriptional response indicative of hypoxia, altering the expression of genes known to respond to low-oxygen conditions. Several additional lines of evidence obtained suggest that these responses could involve effects on heme. First, the hem1Delta mutant lacking the first enzyme in the heme biosynthetic pathway showed increased sensitivity to sampangine, and exogenously supplied hemin partially rescued the inhibitory activity of sampangine in wild-type cells. In addition, heterozygous mutants with deletions in genes involved in five out of eight steps in the heme biosynthetic pathway showed increased susceptibility to sampangine. Furthermore, spectral analyses of pyridine extracts indicated significant accumulation of free porphyrins in sampangine-treated cells. Transcriptional profiling experiments were also performed with C. albicans to investigate the response of a pathogenic fungal species to sampangine. Taking into account the known differences in the physiological responses of C. albicans and S. cerevisiae to low oxygen, significant correlations were observed between the two transcription profiles, suggestive of heme-related defects. Our results indicate that the antifungal activity of the plant alkaloid sampangine is due, at least in part, to perturbations in the biosynthesis or metabolism of heme.
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