ArticleJournal of bacteriology2008
Genetic analysis of lipooligosaccharide core biosynthesis in Campylobacter jejuni 81-176.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Article
- Genetic characterisation of a subset of Campylobacter jejuni isolates from clinical and poultry sources in Ireland.PloS one · 2021Article
- RevisitingFrontiers in cellular and infection microbiology · 2020Review
- An Updated Classification System and Review of the Lipooligosaccharide Biosynthesis Gene Locus inFrontiers in microbiology · 2020Review
- Biosynthesis of GDP-d-Biochemistry · 2019Article
- Evaluation of a conjugate vaccine platform against enterotoxigenic Escherichia coli (ETEC), Campylobacter jejuni and Shigella.Vaccine · 2018Article
- Article
- Glycan:glycan interactions: High affinity biomolecular interactions that can mediate binding of pathogenic bacteria to host cells.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Primary isolation strain determines both phage type and receptors recognised by Campylobacter jejuni bacteriophages.PloS one · 2015Article
- The sweet tooth of bacteria: common themes in bacterial glycoconjugates.Microbiology and molecular biology reviews : MMBR · 2014Review
- The polysaccharide capsule of Campylobacter jejuni modulates the host immune response.Infection and immunity · 2013Article
- Phenotypic and genotypic characterizations of Campylobacter jejuni isolated from the broiler meat production process.Current microbiology · 2012Article
- Cj1136 is required for lipooligosaccharide biosynthesis, hyperinvasion, and chick colonization by Campylobacter jejuni.Infection and immunity · 2012Article
- Effects of sequential Campylobacter jejuni 81-176 lipooligosaccharide core truncations on biofilm formation, stress survival, and pathogenesis.Journal of bacteriology · 2010Article
- Multiple factors interact to produce responses resembling spectrum of human disease in Campylobacter jejuni infected C57BL/6 IL-10-/- mice.BMC microbiology · 2009Article
- Characterization of two Campylobacter jejuni strains for use in volunteer experimental-infection studies.Infection and immunity · 2008Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report isolation and characterization of Campylobacter jejuni 81-176 lgtF and galT lipooligosaccharide (LOS) core mutants. It has been suggested that the lgtF gene of C. jejuni encodes a two-domain glucosyltransferase that is responsible for the transfer of a beta-1,4-glucose residue on heptosyltransferase I (Hep I) and for the transfer of a beta-1,2-glucose residue on Hep II. A site-specific mutation in the lgtF gene of C. jejuni 81-176 resulted in expression of a truncated LOS, and complementation of the mutant in trans restored the core mobility to that of the wild type. Mass spectrometry and nuclear magnetic resonance of the truncated LOS confirmed the loss of two glucose residues, a beta-1,4-glucose on Hep I and a beta-1,2-glucose on Hep II. Mutation of another gene, galT, encoding a glycosyltransferase, which maps outside the region defined as the LOS biosynthetic locus in C. jejuni 81-176, resulted in loss of the beta-(1,4)-galactose residue and all distal residues in the core. Both mutants invaded intestinal epithelial cells in vitro at levels comparable to the wild-type levels, in marked contrast to a deeper inner core waaC mutant. These studies have important implications for the role of LOS in the pathogenesis of Campylobacter-mediated infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.