ArticleJournal of bacteriology2008
Genetic evidence for the actin homolog gene mreBH and the bacitracin resistance gene bcrC as targets of the alternative sigma factor SigI of Bacillus subtilis.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Article
- Genomic Insights into the Pathogenicity and Drug-Resistance of aInfection and drug resistance · 2024Article
- Bacterial SEAL domains undergo autoproteolysis and function in regulated intramembrane proteolysis.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Bacterial SEAL domains undergo autoproteolysis and function in regulated intramembrane proteolysis.bioRxiv : the preprint server for biology · 2023Article
- Peptidoglycan NlpC/P60 peptidases in bacterial physiology and host interactions.Cell chemical biology · 2023Review
- Intrinsically disordered protein regions are required for cell wall homeostasis inGenes & development · 2022Article
- σJournal of bacteriology · 2018Article
- Regulation of biomass degradation by alternative σ factors in cellulolytic clostridia.Scientific reports · 2018Article
- Revisiting the Regulation of the Primary Scaffoldin Gene in Clostridium thermocellum.Applied and environmental microbiology · 2017Article
- The Essential UPP Phosphatase Pair BcrC and UppP Connects Cell Wall Homeostasis during Growth and Sporulation with Cell Envelope Stress Response inFrontiers in microbiology · 2017Article
- YodL and YisK Possess Shape-Modifying Activities That Are Suppressed by Mutations in Bacillus subtilis mreB and mbl.Journal of bacteriology · 2016Article
- Teichoic Acid Polymers Affect Expression and Localization of dl-Endopeptidase LytE Required for Lateral Cell Wall Hydrolysis in Bacillus subtilis.Journal of bacteriology · 2016Article
- Article
- MreB-Dependent Inhibition of Cell Elongation during the Escape from Competence in Bacillus subtilis.PLoS genetics · 2015Article
- FtsEX is required for CwlO peptidoglycan hydrolase activity during cell wall elongation in Bacillus subtilis.Molecular microbiology · 2013Article
- Reexamining transcriptional regulation of the Bacillus subtilis htpX gene and the ykrK gene, encoding a novel type of transcriptional regulator, and redefining the YkrK operator.Journal of bacteriology · 2012Article
- Time-resolved transcriptome analysis of Bacillus subtilis responding to valine, glutamate, and glutamine.PloS one · 2009Article
- The cell wall regulator {sigma}I specifically suppresses the lethal phenotype of mbl mutants in Bacillus subtilis.Journal of bacteriology · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Bacillus subtilis sigI gene, which is a member of the class VI heat shock genes of the B. subtilis heat shock stimulon, encodes an alternative sigma factor whose regulon is poorly defined. In this study, by using a binary vector system, we showed that B. subtilis SigI could drive expression of a transcriptional fusion between the sigI regulatory region from Bacillus licheniformis, Bacillus sp. strain NRRL B-14911, B. subtilis, or Bacillus thuringiensis and the xylE reporter gene in B. subtilis. The transcriptional initiation sites of these fusions in B. subtilis were mapped by primer extension analyses. A putative consensus promoter sequence probably recognized by the B. subtilis SigI was thus deduced. Using a consensus sequence-based search procedure, we found putative sigmaI promoters preceding the actin homolog gene mreBH and the bacitracin resistance gene bcrC of B. subtilis. Overexpression of the B. subtilis sigI gene could specifically stimulate expression of both an mreBH promoter region-bgaB fusion and a bcrC promoter region-bgaB fusion. Expression of these two fusions at the amyE locus of the B. subtilis chromosome was heat inducible and SigI dependent as revealed by sigI gene disruption experiments. Primer extension analysis showed that the identified mreBH and bcrC transcriptional start sites were at appropriate distances from their sigmaI promoter elements. This further supports the notion that SigI can directly regulate mreBH and bcrC expression. Taken together, these results strongly suggest that mreBH and bcrC are new members of the SigI regulon.
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