ArticleJournal of bacteriology2008
Altered utilization of N-acetyl-D-galactosamine by Escherichia coli O157:H7 from the 2006 spinach outbreak.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
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- Harnessing agricultural microbiomes for human pathogen control.ISME communications · 2022Review
- Temporal encoding of bacterial identity and traits in growth dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Draft Genome Sequences of 72 Isolates from All Four Species ofMicrobiology resource announcements · 2018Article
- Factors Involved in the Persistence of a Shiga Toxin-ProducingFrontiers in microbiology · 2018Article
- Comparative Genomic Analysis of Escherichia coli O157:H7 Isolated from Super-Shedder and Low-Shedder Cattle.PloS one · 2016Article
- Article
- Genetic analysis of the roles of agaA, agaI, and agaS genes in the N-acetyl-D-galactosamine and D-galactosamine catabolic pathways in Escherichia coli strains O157:H7 and C.BMC microbiology · 2013Article
- Carbohydrate utilization by enterohaemorrhagic Escherichia coli O157:H7 in bovine intestinal content.Environmental microbiology · 2013Article
- N-acetylgalactosamine utilization pathway and regulon in proteobacteria: genomic reconstruction and experimental characterization in Shewanella.The Journal of biological chemistry · 2012Article
- Genomic anatomy of Escherichia coli O157:H7 outbreaks.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Variability of Escherichia coli O157 strain survival in manure-amended soil in relation to strain origin, virulence profile, and carbon nutrition profile.Applied and environmental microbiology · 2011Article
- The decoupling between genetic structure and metabolic phenotypes in Escherichia coli leads to continuous phenotypic diversity.Journal of evolutionary biology · 2011Article
- The genome sequence of E. coli W (ATCC 9637): comparative genome analysis and an improved genome-scale reconstruction of E. coli.BMC genomics · 2011Article
- Phenotypic diversity of Escherichia coli O157:H7 strains associated with the plasmid O157.Journal of microbiology (Seoul, Korea) · 2010Article
- Phenotype MicroArray in the metabolic characterisation of Salmonella serotypes Agona, Enteritidis, Give, Hvittingfoss, Infantis, Newport and Typhimurium.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2010Article
- Metabolic capacity of Sinorhizobium (Ensifer) meliloti strains as determined by phenotype MicroArray analysis.Applied and environmental microbiology · 2009Article
- Global phenotypic characterization of bacteria.FEMS microbiology reviews · 2009Review
- Important discoveries from analysing bacterial phenotypes.Molecular microbiology · 2008Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In silico analyses of previously sequenced strains of Escherichia coli O157:H7, EDL933 and Sakai, localized the gene cluster for the utilization of N-acetyl-D-galactosamine (Aga) and D-galactosamine (Gam). This gene cluster encodes the Aga phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) and other catabolic enzymes responsible for transport and catabolism of Aga. As the complete coding sequences for enzyme IIA (EIIA)(Aga/Gam), EIIB(Aga), EIIC(Aga), and EIID(Aga) of the Aga PTS are present, E. coli O157:H7 strains normally are able to utilize Aga as a sole carbon source. The Gam PTS complex, in contrast, lacks EIIC(Gam), and consequently, E. coli O157:H7 strains cannot utilize Gam. Phenotypic analyses of 120 independent isolates of E. coli O157:H7 from our culture collection revealed that the overwhelming majority (118/120) displayed the expected Aga+ Gam- phenotype. Yet, when 194 individual isolates, derived from a 2006 spinach-associated E. coli O157:H7 outbreak, were analyzed, all (194/194) displayed an Aga- Gam- phenotype. Comparison of aga/gam sequences from two spinach isolates with those of EDL933 and Sakai revealed a single nucleotide change (G:C-->A:T) in the agaF gene in the spinach-associated isolates. The base substitution in agaF, which encodes EIIA(Aga/Gam) of the PTS, changes a conserved glycine residue to serine (Gly91Ser). Pyrosequencing of this region showed that all spinach-associated E. coli O157:H7 isolates harbored this same G:C-->A:T substitution. Notably, when agaF+ was cloned into an expression vector and transformed into six spinach isolates, all (6/6) were able to grow on Aga, thus demonstrating that the Gly91Ser substitution underlies the Aga- phenotype in these isolates.
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