ArticleJournal of bacteriology2008
Glycerate 2-kinase of Thermotoga maritima and genomic reconstruction of related metabolic pathways.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
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- Controlled Experimental Infection in Pigs with a Strain of Yersinia enterocolitica Harboring Genetic Markers for Human Pathogenicity: Colonization and Stability.Infection and immunity · 2023Article
- Mycobacterial nicotinate mononucleotide adenylyltransferase: structure, mechanism, and implications for drug discovery.The Journal of biological chemistry · 2015Article
- Redox-responsive repressor Rex modulates alcohol production and oxidative stress tolerance in Clostridium acetobutylicum.Journal of bacteriology · 2014Article
- Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.Microbiology and molecular biology reviews : MMBR · 2014Review
- The nitrogen-regulated response regulator NrrA controls cyanophycin synthesis and glycogen catabolism in the cyanobacterium Synechocystis sp. PCC 6803.The Journal of biological chemistry · 2014Article
- Stationary phase and nutrient levels trigger transcription of a genomic locus containing a novel peptide (TM1316) in the hyperthermophilic bacterium Thermotoga maritima.Applied and environmental microbiology · 2013Article
- Functional diversification of ROK-family transcriptional regulators of sugar catabolism in the Thermotogae phylum.Nucleic acids research · 2013Article
- Transcriptional regulation of the carbohydrate utilization network in Thermotoga maritima.Frontiers in microbiology · 2013Article
- Tagaturonate-fructuronate epimerase UxaE, a novel enzyme in the hexuronate catabolic network in Thermotoga maritima.Environmental microbiology · 2012Article
- Diversity and versatility of the Thermotoga maritima sugar kinome.Journal of bacteriology · 2012Article
- Phosphoketolase pathway for xylose catabolism in Clostridium acetobutylicum revealed by 13C metabolic flux analysis.Journal of bacteriology · 2012Article
- N-acetylgalactosamine utilization pathway and regulon in proteobacteria: genomic reconstruction and experimental characterization in Shewanella.The Journal of biological chemistry · 2012Article
- Transcriptional regulation of central carbon and energy metabolism in bacteria by redox-responsive repressor Rex.Journal of bacteriology · 2012Article
- Ribulokinase and transcriptional regulation of arabinose metabolism in Clostridium acetobutylicum.Journal of bacteriology · 2012Article
- Targeting metalloproteins by fragment-based lead discovery.Chemical biology & drug design · 2011Article
- Reconstruction of xylose utilization pathway and regulons in Firmicutes.BMC genomics · 2010Article
- Cohesion group approach for evolutionary analysis of aspartokinase, an enzyme that feeds a branched network of many biochemical pathways.Microbiology and molecular biology reviews : MMBR · 2009Review
- Three-dimensional structural view of the central metabolic network of Thermotoga maritima.Science (New York, N.Y.) · 2009Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Members of a novel glycerate-2-kinase (GK-II) family were tentatively identified in a broad range of species, including eukaryotes and archaea and many bacteria that lack a canonical enzyme of the GarK (GK-I) family. The recently reported three-dimensional structure of GK-II from Thermotoga maritima (TM1585; PDB code 2b8n) revealed a new fold distinct from other known kinase families. Here, we verified the enzymatic activity of TM1585, assessed its kinetic characteristics, and used directed mutagenesis to confirm the essential role of the two active-site residues Lys-47 and Arg-325. The main objective of this study was to apply comparative genomics for the reconstruction of metabolic pathways associated with GK-II in all bacteria and, in particular, in T. maritima. Comparative analyses of approximately 400 bacterial genomes revealed a remarkable variety of pathways that lead to GK-II-driven utilization of glycerate via a glycolysis/gluconeogenesis route. In the case of T. maritima, a three-step serine degradation pathway was inferred based on the tentative identification of two additional enzymes, serine-pyruvate aminotransferase and hydroxypyruvate reductase (TM1400 and TM1401, respectively), that convert serine to glycerate via hydroxypyruvate. Both enzymatic activities were experimentally verified, and the entire pathway was validated by its in vitro reconstitution.
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