ArticleThe Plant cell2007
Regulation of rice NADPH oxidase by binding of Rac GTPase to its N-terminal extension.
Article in The Plant cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 181 papers.
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181 citing papers in PubMed, 459 citations in OpenAlex.
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- Knockout of OsRbohD (the NADPH Oxidase Gene) Enhances Saline-Alkaline Stress Tolerance and Grain Yield in Rice by Reducing ROS Accumulation.Rice (New York, N.Y.) · 2026Article
- Root development progression involves RALF peptide-mediated ROS signaling and pectin dynamics in rice.Nature communications · 2026Article
- Linking Superoxide Production and Scavenging in Plant Development.Plant, cell & environment · 2026Review
- Secreted molecules as modulators of somatic embryogenesis efficiency - an overview.Frontiers in plant science · 2026Review
- Advances in CrRLK1L function in plant cell wall signaling during interaction with the environment and development.Stress biology · 2025Review
- CrWRKY57 and CrABF3 cooperatively activateHorticulture research · 2025Article
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- OsRbohI is the indispensable NADPH oxidase for molecular-patterns-induced reactive oxygen species production in rice.Plant communications · 2024Article
- Rice E3 ubiquitin ligases: From key modulators of host immunity to potential breeding applications.Plant communications · 2024Review
- Genome-Wide Identification, Classification, Expression Analysis, and Screening of Drought and Heat Resistance-Related Candidates of thePlants (Basel, Switzerland) · 2024Article
- A chloroplast localized heavy metal-associated domain containing protein regulates grain calcium accumulation in rice.Nature communications · 2024Article
- Functionality of Reactive Oxygen Species (ROS) in Plants: Toxicity and Control inPlants (Basel, Switzerland) · 2024Review
- RHO OF PLANTS signalling and the activating ROP GUANINE NUCLEOTIDE EXCHANGE FACTORS: specificity in cellular signal transduction in plants.Journal of experimental botany · 2024Review
- Cloning and Functional Analysis of CsROP5 and CsROP10 Genes Involved in Cucumber Resistance toBiology · 2024Article
- Article
121 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reactive oxygen species (ROS) produced by NADPH oxidase play critical roles in various cellular activities, including plant innate immunity response. In contrast with the large multiprotein NADPH oxidase complex of phagocytes, in plants, only the homologs of the catalytic subunit gp91phox and the cytosolic regulator small GTPase Rac are found. Plant homologs of the gp91phox subunit are known as Rboh (for respiratory burst oxidase homolog). Although numerous Rboh have been isolated in plants, the regulation of enzymatic activity remains unknown. All rboh genes identified to date possess a conserved N-terminal extension that contains two Ca2+ binding EF-hand motifs. Previously, we ascertained that a small GTPase Rac (Os Rac1) enhanced pathogen-associated molecular pattern-induced ROS production and resistance to pathogens in rice (Oryza sativa). In this study, using yeast two-hybrid assay, we found that interaction between Rac GTPases and the N-terminal extension is ubiquitous and that a substantial part of the N-terminal region of Rboh, including the two EF-hand motifs, is required for the interaction. The direct Rac-Rboh interaction was supported by further studies using in vitro pull-down assay, a nuclear magnetic resonance titration experiment, and in vivo fluorescence resonance energy transfer (FRET) microscopy. The FRET analysis also suggests that cytosolic Ca2+ concentration may regulate Rac-Rboh interaction in a dynamic manner. Furthermore, transient coexpression of Os Rac1 and rbohB enhanced ROS production in Nicotiana benthamiana, suggesting that direct Rac-Rboh interaction may activate NADPH oxidase activity in plants. Taken together, the results suggest that cytosolic Ca2+ concentration may modulate NADPH oxidase activity by regulating the interaction between Rac GTPase and Rboh.
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