ArticleJournal of molecular biology2008
Assembly of the 5' and 3' minor domains of 16S ribosomal RNA as monitored by tethered probing from ribosomal protein S20.
Article in Journal of molecular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Mechanism of 30S subunit recognition and modification by the conserved bacterial ribosomal RNA methyltransferase RsmI.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Article
- Extraribosomal Functions of Bacterial Ribosomal Proteins-An Update, 2023.International journal of molecular sciences · 2024Review
- Late consolidation of rRNA structure during co-transcriptional assembly inbioRxiv : the preprint server for biology · 2024Article
- KsgA facilitates ribosomal small subunit maturation by proofreading a key structural lesion.Nature structural & molecular biology · 2023Article
- Structural Insights into the Distortion of the Ribosomal Small Subunit at Different Magnesium Concentrations.Biomolecules · 2023Article
- A conserved rRNA switch is central to decoding site maturation on the small ribosomal subunit.Science advances · 2021Article
- Dissecting macromolecular recognition sites in ribosome: implication to its self-assembly.RNA biology · 2019Article
- A metastable rRNA junction essential for bacterial 30S biogenesis.Nucleic acids research · 2018Article
- Structure and dynamics of bacterial ribosome biogenesis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Differential effects of ribosomal proteins and Mg2+ ions on a conformational switch during 30S ribosome 5'-domain assembly.RNA (New York, N.Y.) · 2015Article
- Functions of ribosomal proteins in assembly of eukaryotic ribosomes in vivo.Annual review of biochemistry · 2015Review
- Structural insights into the assembly of the 30S ribosomal subunit in vivo: functional role of S5 and location of the 17S rRNA precursor sequence.Protein & cell · 2014Article
- Cryo-EM structures of the late-stage assembly intermediates of the bacterial 50S ribosomal subunit.Nucleic acids research · 2013Article
- Dissecting the in vivo assembly of the 30S ribosomal subunit reveals the role of RimM and general features of the assembly process.Nucleic acids research · 2013Article
- A computational investigation on the connection between dynamics properties of ribosomal proteins and ribosome assembly.PLoS computational biology · 2012Article
- Interdependencies govern multidomain architecture in ribosomal small subunit assembly.RNA (New York, N.Y.) · 2011Article
- Global stabilization of rRNA structure by ribosomal proteins S4, S17, and S20.Journal of molecular biology · 2009Article
- Nob1 binds the single-stranded cleavage site D at the 3'-end of 18S rRNA with its PIN domain.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The ribosomal protein (r-protein) S20 is a primary binding protein. As such, it interacts directly and independently with the 5' domain as well as the 3' minor domain of 16S ribosomal RNA (rRNA) in minimal particles and the fully assembled 30S subunit. The interactions observed between r-protein S20 and the 5' domain of 16S rRNA are quite extensive, while those between r-protein S20 and the 3' minor domain are significantly more limited. In this study, directed hydroxyl radical probing mediated by Fe(II)-derivatized S20 proteins was used to monitor the folding of 16S rRNA during r-protein association and 30S subunit assembly. An analysis of the cleavage patterns in the minimal complexes [16S rRNA and Fe(II)-S20] and the fully assembled 30S subunit containing the same Fe(II)-derivatized proteins shows intriguing similarities and differences. These results suggest that the two domains, 5' and 3' minor, are organized relative to S20 at different stages of assembly. The 5' domain acquires, in a less complex ribonucleoprotein particle than the 3' minor domain, the same architecture as observed in mature subunits. These results are similar to what would be predicted of subunit assembly by the 5'-to-3' direction assembly model.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.