ArticleBiophysical journal2008
Yeast ribosomal stalk heterogeneity in vivo shown by two-photon FCS and molecular brightness analysis.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Interplay Between Ribosomal Gene Deficiency and Calorie Restriction in Shaping Yeast Biosynthetic Capacity.Cells · 2025Article
- CHIRP-Seq: FOXP2 transcriptional targets in zebra finch brain include numerous speech and language-related genes.BMC neuroscience · 2025Article
- Kaposi's sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs.Nature communications · 2023Article
- Use of red, far-red, and near-infrared light in imaging of yeasts and filamentous fungi.Applied microbiology and biotechnology · 2022Review
- Ribosome heterogeneity in Drosophila melanogaster gonads through paralog-switching.Nucleic acids research · 2022Article
- Ribosome heterogeneity and specialization in development.Wiley interdisciplinary reviews. RNA · 2021Review
- Quantifying the Oligomeric State of hZIP4 on the Surface of Cells.Biochemistry · 2019Article
- Scanning number and brightness yields absolute protein concentrations in live cells: a crucial parameter controlling functional bio-molecular interaction networks.Biophysical reviews · 2018Review
- Functional divergence between the two P1-P2 stalk dimers on the ribosome in their interaction with ricin A chain.The Biochemical journal · 2014Article
- Targeting ricin to the ribosome.Toxicon : official journal of the International Society on Toxinology · 2013Review
- Laurdan generalized polarization fluctuations measures membrane packing micro-heterogeneity in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- P1 and P2 protein heterodimer binding to the P0 protein of Saccharomyces cerevisiae is relatively non-specific and a source of ribosomal heterogeneity.Nucleic acids research · 2012Article
- Alpha hemolysin induces an increase of erythrocytes calcium: a FLIM 2-photon phasor analysis approach.PloS one · 2011Article
- Lipid packing determines protein-membrane interactions: challenges for apolipoprotein A-I and high density lipoproteins.Biochimica et biophysica acta · 2010Review
- Fluorescence fluctuation spectroscopy: ushering in a new age of enlightenment for cellular dynamics.Biophysical reviews · 2009Article
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Authors and funding
8 authors.
Funding
Abstract
The stalk of Saccharomyces cerevisiae ribosomes contains, on average, five distinct proteins, namely P0 and four acidic proteins, P1alpha, P1beta, P2alpha, and P2beta. Each ribosome contains only one copy of P0, but the distribution of the acidic proteins among the ribosome population in vivo has not been determined. Using two-photon fluorescence correlation spectroscopy and scanning FCS, on cells expressing EGFP-tagged P0, P1, and P2 proteins, we show, with brightness analysis, that individual yeast ribosomes in vivo are compositionally heterogeneous in regard to P1alpha, P1beta, P2alpha, and P2beta. These results are relevant to the hypothesis, based on in vitro studies, that the overall cellular pattern of expressed proteins can be determined by the distribution of the stalk proteins among the ribosome population.
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Registered trials
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