ArticleNucleic acids research2008
Targeted induction of meiotic double-strand breaks reveals chromosomal domain-dependent regulation of Spo11 and interactions among potential sites of meiotic recombination.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 29 citations in OpenAlex.
- Mutual, spatially limited control of meiotic DNA break formation by Mre11-Rad50-Nbs1 DNA repair complex and Tel1 (ATM) protein kinase.Nucleic acids research · 2025Article
- Molecular mechanisms and regulation of recombination frequency and distribution in plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- Chromosome-dependent aneuploid formation in Spo11-less meiosis.Genes to cells : devoted to molecular & cellular mechanisms · 2023Article
- Redirecting meiotic DNA break hotspot determinant proteins alters localized spatial control of DNA break formation and repair.Nucleic acids research · 2022Article
- Physical basis for long-distance communication along meiotic chromosomes.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Pericentromere-Specific Cohesin Complex Prevents Meiotic Pericentric DNA Double-Strand Breaks and Lethal Crossovers.Molecular cell · 2018Article
- Article
- Numerical and spatial patterning of yeast meiotic DNA breaks by Tel1.Genome research · 2017Article
- Article
- Repression of harmful meiotic recombination in centromeric regions.Seminars in cell & developmental biology · 2016Review
- Meiotic DSB patterning: A multifaceted process.Cell cycle (Georgetown, Tex.) · 2016Review
- The CAF-1 and Hir Histone Chaperones Associate with Sites of Meiotic Double-Strand Breaks in Budding Yeast.PloS one · 2015Article
- Mechanism and regulation of meiotic recombination initiation.Cold Spring Harbor perspectives in biology · 2014Review
- Condensin suppresses recombination and regulates double-strand break processing at the repetitive ribosomal DNA array to ensure proper chromosome segregation during meiosis in budding yeast.Molecular biology of the cell · 2014Article
- Self-organization of meiotic recombination initiation: general principles and molecular pathways.Annual review of genetics · 2014Review
- Suppression of genetic recombination in the pseudoautosomal region and at subtelomeres in mice with a hypomorphic Spo11 allele.BMC genomics · 2013Article
- Meiotic double-strand breaks occur once per pair of (sister) chromatids and, via Mec1/ATR and Tel1/ATM, once per quartet of chromatids.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Many functions of the meiotic cohesin.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Review
- Genetics of mammalian meiosis: regulation, dynamics and impact on fertility.Nature reviews. Genetics · 2010Review
- Rec8 guides canonical Spo11 distribution along yeast meiotic chromosomes.Molecular biology of the cell · 2009Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Meiotic recombination is initiated by programmed DNA double-strand break (DSB) formation mediated by Spo11. DSBs occur with frequency in chromosomal regions called hot domains but are seldom seen in cold domains. To obtain insights into the determinants of the distribution of meiotic DSBs, we examined the effects of inducing targeted DSBs during yeast meiosis using a UAS-directed form of Spo11 (Gal4BD-Spo11) and a meiosis-specific endonuclease, VDE (PI-SceI). Gal4BD-Spo11 cleaved its target sequence (UAS) integrated in hot domains but rarely in cold domains. However, Gal4BD-Spo11 did bind to UAS and VDE efficiently cleaved its recognition sequence in either context, suggesting that a cold domain is not a region of inaccessible or uncleavable chromosome structure. Importantly, self-association of Spo11 occurred at UAS in a hot domain but not in a cold domain, raising the possibility that Spo11 remains in an inactive intermediate state in cold domains. Integration of UAS adjacent to known DSB hotspots allowed us to detect competitive interactions among hotspots for activation. Moreover, the presence of VDE-introduced DSB repressed proximal hotspot activity, implicating DSBs themselves in interactions among hotspots. Thus, potential sites for Spo11-mediated DSB are subject to domain-specific and local competitive regulations during and after DSB formation.
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