ArticleNucleic acids research2008
Site-specific recombination in Schizosaccharomyces pombe and systematic assembly of a 400kb transgene array in mammalian cells using the integrase of Streptomyces phage phiBT1.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 33 citations in OpenAlex.
- Efficient biosynthesis of transglutaminase inCurrent research in food science · 2024Article
- Application of serine integrases for secondary metabolite pathway assembly inSynthetic and systems biotechnology · 2020Article
- Human Artificial Chromosome with Regulated Centromere: A Tool for Genome and Cancer Studies.ACS synthetic biology · 2018Review
- Method to Assemble Genomic DNA Fragments or Genes on Human Artificial Chromosome with Regulated Kinetochore Using a Multi-Integrase System.ACS synthetic biology · 2018Article
- Logic Synthesis of Recombinase-Based Genetic Circuits.Scientific reports · 2017Article
- Genome Integration and Excision by a New Streptomyces Bacteriophage, ϕJoe.Applied and environmental microbiology · 2017Article
- Development of a novel HAC-based "gain of signal" quantitative assay for measuring chromosome instability (CIN) in cancer cells.Oncotarget · 2016Article
- New applications for phage integrases.Journal of molecular biology · 2014Review
- Kinetochore assembly and heterochromatin formation occur autonomously in Schizosaccharomyces pombe.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome.BMC biotechnology · 2013Article
- Control of directionality in Streptomyces phage φBT1 integrase-mediated site-specific recombination.PloS one · 2013Article
- Streptomyces temperate bacteriophage integration systems for stable genetic engineering of actinomycetes (and other organisms).Journal of industrial microbiology & biotechnology · 2012Review
- Controlled somatic and germline copy number variation in the mouse model.Current genomics · 2010Article
- Highly efficient in vitro site-specific recombination system based on streptomyces phage phiBT1 integrase.Journal of bacteriology · 2008Article
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Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
We have established the integrase of the Streptomyces phage phiBT1 as a tool for eukaryotic genome manipulation. We show that the phiBT1 integrase promotes efficient reciprocal and conservative site-specific recombination in vertebrate cells and in Schizosaccharomyces pombe, thus establishing the utility of this protein for genome manipulation in a wide range of eukaryotes. We show that the phiBT1 integrase can be used in conjunction with Cre recombinase to promote the iterative integration of transgenic DNA. We describe five cycles of iterative integration of a candidate mouse centromeric sequence 80 kb in length into a human mini-chromosome within a human-Chinese hamster hybrid cell line. These results establish the generality of the iterative site-specific integration technique.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.