ArticleBMC molecular biology2007
Identifying the most suitable endogenous control for determining gene expression in hearts from organ donors.
Article in BMC molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Screening of Reference Gene for RT-qPCR inInternational journal of molecular sciences · 2026Article
- Advancements in Reference Gene Selection for Fruit Trees: A Comprehensive Review.International journal of molecular sciences · 2024Review
- Evaluation of a Porcine Endogenous Reference Gene (Internal Sample Control) in a Porcine Reproductive and Respiratory Syndrome Virus RT-qPCR.Veterinary sciences · 2023Article
- Reference Genes Selection ofJournal of fungi (Basel, Switzerland) · 2022Article
- Stable reference gene selection for quantitative real-time PCR normalization in passion fruit (Passiflora edulis Sims.).Molecular biology reports · 2022Article
- Normalization strategy for selection of reference genes for RT-qPCR analysis in left ventricles of failing human hearts.BMC cardiovascular disorders · 2022Article
- Sexual-biased gene expression of olfactory-related genes in the antennae of Conogethes pinicolalis (Lepidoptera: Crambidae).BMC genomics · 2020Article
- Selection of suitable reference genes for core clock gene expression analysis by real-time qPCR in rat ovary granulosa cells.Molecular biology reports · 2019Article
- Article
- Protein and mRNA expression of folic acid-associated enzymes as biomarkers for the cytotoxicity of the thymidylate synthase-targeted drugs, pemetrexed and S-1, in non-small cell lung cancer.Molecular and clinical oncology · 2017Article
- Impact of insulin-like growth factor-1 receptor and amphiregulin expression on survival in patients with stage II/III gastric cancer enrolled in the Adjuvant Chemotherapy Trial of S-1 for Gastric Cancer.Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association · 2017Article
- Selection of reference genes is critical for miRNA expression analysis in human cardiac tissue. A focus on atrial fibrillation.Scientific reports · 2017Article
- Training differentially regulates elastin level and proteolysis in skeletal and heart muscles and aorta in healthy rats.Biology open · 2016Article
- Correlation between S-1 treatment outcome and expression of biomarkers for refractory thymic carcinoma.BMC cancer · 2016Article
- Gapdh gene expression is modulated by inflammatory arthritis and is not suitable for qPCR normalization.Inflammation · 2014Article
- Transcriptome Analysis of Spermophilus lateralis and Spermophilus tridecemlineatus Liver Does Not Suggest the Presence of Spermophilus-Liver-Specific Reference Genes.ISRN bioinformatics · 2013Article
- Validation of reference genes for gene expression studies in peanut by quantitative real-time RT-PCR.Molecular genetics and genomics : MGG · 2012Article
- Selection of reference genes for quantitative real-time RT-PCR analysis in citrus.Molecular biology reports · 2012Article
- UBC and YWHAZ as suitable reference genes for accurate normalisation of gene expression using MCF7, HCT116 and HepG2 cell lines.Cytotechnology · 2011Article
- Article
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Authors and funding
8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundQuantitative real-time reverse transcription PCR (qRT-PCR) is a useful tool for assessing gene expression in different tissues, but the choice of adequate controls is critical to normalise the results, thereby avoiding differences and maximizing sensitivity and accuracy. So far, many genes have been used as a single reference gene, without having previously verified their value as controls. This practice can lead to incorrect conclusions and recent evidence indicates a need to use the geometric mean of data from several control genes. Here, we identified an appropriate set of genes to be used as an endogenous reference for quantifying gene expression in human heart tissue.
resultsOur findings indicate that out of ten commonly used reference genes (GADPH, PPIA, ACTB, YWHAZ, RRN18S, B2M, UBC, TBP, RPLP and HPRT), PPIA, RPLP and GADPH show the most stable gene transcription levels in left ventricle specimens obtained from organ donors, as assessed using geNorm and Normfinder software. The expression of TBP was found to be highly regulated.
conclusionWe propose the use of PPIA, RPLP and GADPH as reference genes for the accurate normalisation of qRT-PCR performed on heart tissue. TBP should not be used as a control in this type of tissue.
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