ArticleProtoplasma2007
Actin and myosin inhibitors block elongation of kinetochore fibre stubs in metaphase crane-fly spermatocytes.
Article in Protoplasma, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Blocking microtubule deacetylation inhibits anaphase chromosome movements in crane-fly spermatocytes.PloS one · 2024Article
- Artificially decreasing cortical tension generates aneuploidy in mouse oocytes.Nature communications · 2020Article
- Hypothesis-driven research for hypothesis-driven application.Protoplasma · 2015Article
- Movement of chromosomes with severed kinetochore microtubules.Protoplasma · 2015Article
- Gamma-actin is involved in regulating centrosome function and mitotic progression in cancer cells.Cell cycle (Georgetown, Tex.) · 2015Article
- Targeting of several glycolytic enzymes using RNA interference reveals aldolase affects cancer cell proliferation through a non-glycolytic mechanism.The Journal of biological chemistry · 2012Article
- Do nuclear envelope and intranuclear proteins reorganize during mitosis to form an elastic, hydrogel-like spindle matrix?Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Review
- The perpetual movements of anaphase.Cellular and molecular life sciences : CMLS · 2010Review
- Article
- Abundance of actin filaments in the preprophase band and mitotic spindle of brick1 Zea mays mutant.Protoplasma · 2009Article
- Mitosis: spindle evolution and the matrix model.Protoplasma · 2009Article
- Myosin-10 and actin filaments are essential for mitotic spindle function.The Journal of cell biology · 2008Article
- What generates flux of tubulin in kinetochore microtubules?Protoplasma · 2008Article
- The vertebrate cell kinetochore and its roles during mitosis.Trends in cell biology · 1998Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We used an ultraviolet microbeam to cut individual kinetochore spindle fibres in metaphase crane-fly spermatocytes. We then followed the growth of the "kinetochore stubs", the remnants of kinetochore fibres that remain attached to kinetochores. Kinetochore stubs elongate with constant velocity by adding tubulin subunits at the kinetochore, and thus elongation is related to tubulin flux in the kinetochore microtubules. Stub elongation was blocked by cytochalasin D and latrunculin A, actin inhibitors, and by butanedione monoxime, a myosin inhibitor. We conclude that actin and myosin are involved in generating elongation and thus in producing tubulin flux in kinetochore microtubules. We suggest that actin and myosin act in concert with a spindle matrix to propel kinetochore fibres poleward, thereby causing stub elongation and generating anaphase chromosome movement in nonirradiated cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.