ArticleProtoplasma2007
Heterologous expression of vacuolar H(+)-PPase enhances the electrochemical gradient across the vacuolar membrane and improves tobacco cell salt tolerance.
Article in Protoplasma, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 49 citations in OpenAlex.
- UBIQUITIN-CONJUGATING ENZYME34 mediates pyrophosphatase AVP1 turnover and regulates abiotic stress responses in Arabidopsis.Plant physiology · 2025Article
- Gene pyramiding for boosted plant growth and broad abiotic stress tolerance.Plant biotechnology journal · 2024Article
- Whole Genome Duplication Events Likely Contributed to the Aquatic Adaptive Evolution of Parkerioideae.Plants (Basel, Switzerland) · 2024Article
- Seagrass genomes reveal ancient polyploidy and adaptations to the marine environment.Nature plants · 2024Article
- Elemental profiling and genome-wide association studies reveal genomic variants modulating ionomic composition inFrontiers in plant science · 2024Article
- The proteome landscape of the root cap reveals a role for the jacalin-associated lectin JAL10 in the salt-induced endoplasmic reticulum stress pathway.Plant communications · 2023Article
- Phenotypic characterization ofApplications in plant sciences · 2020Article
- High V-PPase activity is beneficial under high salt loads, but detrimental without salinity.The New phytologist · 2018Article
- Article
- Characterization and expression analyses of the H⁺-pyrophosphatase gene in rye.Journal of genetics · 2016Article
- Physiological and molecular mechanisms of plant salt tolerance.Photosynthesis research · 2013Review
- Expression of wheat Na(+)/H(+) antiporter TNHXS1 and H(+)- pyrophosphatase TVP1 genes in tobacco from a bicistronic transcriptional unit improves salt tolerance.Plant molecular biology · 2012Article
- Combining enhanced root and shoot growth reveals cross talk between pathways that control plant organ size in Arabidopsis.Plant physiology · 2011Article
- The yeast CLC protein counteracts vesicular acidification during iron starvation.Journal of cell science · 2010Article
- A large insert Thellungiella halophila BIBAC library for genomics and identification of stress tolerance genes.Plant molecular biology · 2010Article
- Learning from evolution: Thellungiella generates new knowledge on essential and critical components of abiotic stress tolerance in plants.Molecular plant · 2009Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The vacuolar H(+)-translocating inorganic pyrophosphatase (H(+)-PPase) uses pyrophosphate as substrate to generate the proton electrochemical gradient across the vacuolar membrane to acidify vacuoles in plant cells. The heterologous expression of H(+)-PPase genes (TsVP from Thellungiella halophila and AVP1 from Arabidopsis thaliana) improved the salt tolerance of tobacco plants. Under salt stress, the transgenic seedlings showed much better growth and greater fresh weight than wild-type plants, and their protoplasts had a normal appearance and greater vigor. The cytoplasmic and vacuolar pH in transgenic and wild-type cells were measured with a pH-sensitive fluorescence indicator. The results showed that heterologous expression of H(+)-PPase produced an enhanced proton electrochemical gradient across the vacuolar membrane, which accelerated the sequestration of sodium ions into the vacuole. More Na(+) accumulated in the vacuoles of transgenic cells under salt (NaCl) stress, revealed by staining with the fluorescent indicator Sodium Green. It was concluded that the tonoplast-resident H(+)-PPase plays important roles in the maintenance of the proton gradient across the vacuolar membrane and the compartmentation of Na(+) within vacuoles, and heterologous expression of this protein enhanced the electrochemical gradient across the vacuolar membrane, thereby improving the salt tolerance of tobacco cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.