ArticleLab on a chip2008
Controlled on-chip stimulation of quantal catecholamine release from chromaffin cells using photolysis of caged Ca2+ on transparent indium-tin-oxide microchip electrodes.
Article in Lab on a chip, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- Methodologies for Detecting Quantal Exocytosis in Adrenal Chromaffin Cells Through Diamond-Based MEAs.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Surface-modified CMOS IC electrochemical sensor array targeting single chromaffin cells for highly parallel amperometry measurements.Pflugers Archiv : European journal of physiology · 2018Review
- Electrochemical measurement of quantal exocytosis using microchips.Pflugers Archiv : European journal of physiology · 2018Review
- All-carbon multi-electrode array for real-time in vitro measurements of oxidizable neurotransmitters.Scientific reports · 2016Article
- Two approaches for addressing electrochemical electrode arrays with reduced external connections.Analytical methods : advancing methods and applications · 2015Article
- Development and characterization of a diamond-insulated graphitic multi electrode array realized with ion beam lithography.Sensors (Basel, Switzerland) · 2014Article
- Spatial resolution of single-cell exocytosis by microwell-based individually addressable thin film ultramicroelectrode arrays.Analytical chemistry · 2014Article
- Electroporation followed by electrochemical measurement of quantal transmitter release from single cells using a patterned microelectrode.Lab on a chip · 2013Article
- Quantification of noise sources for amperometric measurement of quantal exocytosis using microelectrodes.The Analyst · 2012Article
- Transparent Electrode Materials for Simultaneous Amperometric Detection of Exocytosis and Fluorescence Microscopy.Journal of biomaterials and nanobiotechnology · 2012Article
- Chemical analysis of single cells.Analytical chemistry · 2011Review
- Microwell device for targeting single cells to electrochemical microelectrodes for high-throughput amperometric detection of quantal exocytosis.Analytical chemistry · 2011Article
- Automated targeting of cells to electrochemical electrodes using a surface chemistry approach for the measurement of quantal exocytosis.ACS chemical neuroscience · 2010Article
- Electrically evoking and electrochemically resolving quantal release on a microchip.Lab on a chip · 2010Article
- Improved surface-patterned platinum microelectrodes for the study of exocytotic events.Analytical chemistry · 2009Article
- Preferential cell attachment to nitrogen-doped diamond-like carbon (DLC:N) for the measurement of quantal exocytosis.Biomaterials · 2009Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Photorelease of caged Ca(2+) is a uniquely powerful tool to study the dynamics of Ca(2+)-triggered exocytosis from individual cells. Using photolithography and other microfabrication techniques, we have developed transparent microchip devices to enable photorelease of caged Ca(2+), together with electrochemical detection of quantal catecholamine secretion from individual cells or cell arrays as a step towards developing high-throughput experimental devices. A 100 nm thick transparent indium-tin-oxide (ITO) film was sputter-deposited onto glass coverslips, which were then patterned into 24 cell-sized working electrodes (approximately 20 microm by 20 microm). We loaded bovine chromaffin cells with acetoxymethyl (AM) ester derivatives of the Ca(2+) cage NP-EGTA and Ca(2+) indicator dye fura-4F, then transferred these cells onto the working ITO electrodes for amperometric recordings. Upon flash photorelease of caged Ca(2+), a uniform rise of [Ca(2+)](i) within the target cell leads to quantal release of oxidizable catecholamines measured amperometrically by the underlying ITO electrode. We observed a burst of amperometric spikes upon rapid elevation of [Ca(2+)](i) and a "priming" effect of sub-stimulatory [Ca(2+)](i) on the response of cells to subsequent [Ca(2+)](i) elevation, similar to previous reports using different techniques. We conclude that UV photolysis of caged Ca(2+) is a suitable stimulation technique for higher-throughput studies of Ca(2+)-dependent exocytosis on transparent electrochemical microelectrode arrays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.