ArticleJournal of virology2008
Fusogenicity of Jaagsiekte sheep retrovirus envelope protein is dependent on low pH and is enhanced by cytoplasmic tail truncations.
Article in Journal of virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Enzootic nasal tumor virus type 2 envelope of goats acts as a retroviral oncogene in cell transformation.Virus genes · 2021Article
- The U3 and Env Proteins of Jaagsiekte Sheep Retrovirus and Enzootic Nasal Tumor Virus Both Contribute to Tissue Tropism.Viruses · 2019Article
- Article
- Induction of Cell-Cell Fusion by Ebola Virus Glycoprotein: Low pH Is Not a Trigger.PLoS pathogens · 2016Article
- A sorting signal suppresses IFITM1 restriction of viral entry.The Journal of biological chemistry · 2015Article
- The cytoplasmic tail of retroviral envelope glycoproteins.Progress in molecular biology and translational science · 2015Review
- TIM-family proteins inhibit HIV-1 release.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Structural and functional comparisons of retroviral envelope protein C-terminal domains: still much to learn.Viruses · 2014Review
- IFITM proteins restrict viral membrane hemifusion.PLoS pathogens · 2013Article
- The N-terminal region of IFITM3 modulates its antiviral activity by regulating IFITM3 cellular localization.Journal of virology · 2012Article
- Critical role of leucine-valine change in distinct low pH requirements for membrane fusion between two related retrovirus envelopes.The Journal of biological chemistry · 2012Article
- Membrane fusion and cell entry of XMRV are pH-independent and modulated by the envelope glycoprotein's cytoplasmic tail.PloS one · 2012Article
- Cell entry of enveloped viruses.Advances in genetics · 2011Review
- Reconstitution of the ancestral glycoprotein of human endogenous retrovirus k and modulation of its functional activity by truncation of the cytoplasmic domain.Journal of virology · 2009Article
- Receptor binding and low pH coactivate oncogenic retrovirus envelope-mediated fusion.Journal of virology · 2009Article
- Friendly viruses: the special relationship between endogenous retroviruses and their host.Annals of the New York Academy of Sciences · 2009Article
- Enzootic nasal tumor virus envelope requires a very acidic pH for fusion activation and infection.Journal of virology · 2008Article
- Jaagsiekte sheep retrovirus utilizes a pH-dependent endocytosis pathway for entry.Journal of virology · 2008Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Jaagsiekte sheep retrovirus (JSRV) envelope (Env) is an active oncogene responsible for neoplastic transformation in animals and cultured cells. In this study, we used syncytium induction and fluorescence-based cell fusion assays to investigate JSRV Env fusion and its modulation by the cytoplasmic tail (CT). We found that JSRV Env induced syncytia in cells overexpressing the receptor for JSRV and that a low pH was required for this process to occur. Fusion kinetics studies revealed that cell-cell fusion by JSRV Env at neutral pH was poor, taking up to a day, in sharp contrast to fusion at low pH, which peaked within 2 min following a low-pH trigger. Deletion of the C-terminal 7 or 16 amino acids of the JSRV Env CT had no or little effect on fusion, yet additional truncation toward the membrane-spanning domain, resulting in mutants retaining as little as 1 amino acid of the CT, led to progressively increased syncytium formation at neutral pH that was further enhanced by low-pH treatment. Notably, the severely truncated mutants showed elevated levels of surface subunits in culture medium, suggesting that the CT truncations resulted in conformational changes in the ectodomain of Env that impaired surface subunit associations. Taken together, this study reveals for the first time that the fusion activity of the JSRV Env protein is dependent on a low pH and is modulated by the CT, whose truncation overcomes, at least partially, the low-pH requirement for fusion and enhances Env fusion activity and kinetics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.