ArticleJournal of clinical microbiology2008
Utility of real-time PCR for diagnosis of Legionnaires' disease in routine clinical practice.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03200275 (The Impact of Legionella Urine Antigen Testing), which is not on this map. Cited by 23 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
The Impact of Legionella Urine Antigen Testing (LUAT) on the Local Epidemiology and Diagnosis of Legionella Pneumonia - A Hospital Based Study in Malaysia
Who cites it
23 citing papers in PubMed, 2 syntheses or guidelines pooled it, 88 citations in OpenAlex.
- Diagnostic Accuracy of PCR Alone and Compared to Urinary Antigen Testing for Detection of Legionella spp.: a Systematic Review.Journal of clinical microbiology · 2016Pooled it
- Guidelines for the management of adult lower respiratory tract infections--full version.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2011Guideline
- The Adequacy of Current Legionnaires' Disease Diagnostic Practices in Capturing the Epidemiology of Clinically RelevantPathogens (Basel, Switzerland) · 2024Article
- Incidence and risk factors associated with progression to severe pneumonia among adults with non-severe Legionella pneumonia.Acute and critical care · 2022Article
- Multiple Cross Displacement Amplification Coupled with Lateral Flow Biosensor (MCDA-LFB) for rapid detection of Legionella pneumophila.BMC microbiology · 2022Article
- A Hospital-based Study on the Local Epidemiology of Pneumonia Including the Contribution of Legionella Pneumonia.The Malaysian journal of medical sciences : MJMS · 2020Article
- Comparison of in situ sequence type analysis of Legionella pneumophila in respiratory tract secretions and environmental samples of a hospital in East Jerusalem.Epidemiology and infection · 2018Article
- Severe pneumonitis refractory to steroids following anti-PD-1 immunotherapy.BMJ case reports · 2018Article
- Article
- Rare presentation of an old bug.BMJ case reports · 2017Article
- Diagnostic testing for Legionnaires' disease.Annals of clinical microbiology and antimicrobials · 2017Review
- Evaluation of Patients with Community-Acquired Pneumonia Caused by Zoonotic Pathogens in an Area with a High Density of Animal Farms.Zoonoses and public health · 2016Article
- How recent advances in molecular tests could impact the diagnosis of pneumonia.Expert review of molecular diagnostics · 2016Review
- Utility of PCR, Culture, and Antigen Detection Methods for Diagnosis of Legionellosis.Journal of clinical microbiology · 2015Article
- Current and emerging Legionella diagnostics for laboratory and outbreak investigations.Clinical microbiology reviews · 2015Review
- Evaluation of yield of currently available diagnostics by sample type to optimize detection of respiratory pathogens in patients with a community-acquired pneumonia.Influenza and other respiratory viruses · 2014Observational
- Viral and bacterial aetiology of community-acquired pneumonia in adults.Influenza and other respiratory viruses · 2013Article
- Role of molecular diagnostics in the management of infectious disease emergencies.The Medical clinics of North America · 2012Review
- Specific real-time PCR for simultaneous detection and identification of Legionella pneumophila serogroup 1 in water and clinical samples.Applied and environmental microbiology · 2011Article
- Community-acquired pneumonia due to Legionella pneumophila, the utility of PCR, and a review of the antibiotics used.International journal of general medicine · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The main aim of our study was to determine the added value of PCR for the diagnosis of Legionnaires' disease (LD) in routine clinical practice. The specimens were samples submitted for routine diagnosis of pneumonia from December 2002 to November 2005. Patients were evaluated if, in addition to PCR, the results of at least one of the following diagnostic tests were available: (i) culture for Legionella spp. on buffered charcoal yeast extract agar or (ii) detection of Legionella pneumophila antigen in urine specimens. Of the 151 evaluated patients, 37 (25%) fulfilled the European Working Group on Legionella Infections criteria for a confirmed case of LD (the "gold standard"). An estimated sensitivity, specificity, and overall percent agreement of 86% (32 of 37; 95% confidence interval [CI] = 72 to 95%), 95% (107 of 112; 95% CI = 90 to 98%), and 93% (139 of 149), respectively, were found for 16S rRNA-based PCR, and corresponding values of 92% (34 of 37; 95% CI = 78 to 98%), 98% (110 of 112; 95% CI = 93 to 100%), and 97% (144 of 149), respectively, were found for the mip gene-based PCR. A total of 35 patients were diagnosed by using the urinary antigen test, and 34 were diagnosed by the 16S rRNA-based PCR. With the mip gene PCR one more case of LD (n = 36; not significant) was detected. By combining urinary antigen test and the mip gene PCR, LD was diagnosed in an additional 4 (11%) patients versus the use of the urinary antigen test alone. The addition of a L. pneumophila-specific mip gene PCR to a urinary antigen test is useful in patients with suspected LD who produce sputum and might allow the early detection of a significant number of additional patients.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.