ArticleJournal of clinical microbiology2008
Simultaneous detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae by use of molecular beacons in a duplex real-time PCR.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 40 citations in OpenAlex.
- Post-pandemic surge ofJournal of clinical microbiology · 2026Article
- ERA-CRISPR/Cas12a-based, fast and specific diagnostic detection forFrontiers in cellular and infection microbiology · 2024Article
- Recombinase Polymerase Amplification Combined with Real-Time Fluorescent Probe forJournal of clinical medicine · 2022Article
- The crosstalk between microbiome and asthma: Exploring associations and challenges.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2019Review
- Detection ofThe American journal of tropical medicine and hygiene · 2017Article
- No detection of macrolide-resistant Mycoplasma pneumoniae from Swedish patients, 1996-2013.Infection ecology & epidemiology · 2016Article
- Development and laboratory evaluation of a real-time PCR assay for detecting viruses and bacteria of relevance for community-acquired pneumonia.The Journal of molecular diagnostics : JMD · 2015Article
- Article
- Bacterial complications of respiratory tract viral illness: a comprehensive evaluation.The Journal of infectious diseases · 2013Article
- Molecular detection of Mycoplasma pneumoniae by quantitative real-time PCR in patients with community acquired pneumonia.The Indian journal of medical research · 2013Article
- Utility of serum procalcitonin values in patients with acute exacerbations of chronic obstructive pulmonary disease: a cautionary note.International journal of chronic obstructive pulmonary disease · 2012Article
- Detection of Mycoplasma pneumoniae in different respiratory specimens.European journal of pediatrics · 2011Article
- Detection of Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella spp. in clinical specimens using a single-tube multiplex real-time PCR assay.Diagnostic microbiology and infectious disease · 2011Article
- Acute respiratory infection due to Mycoplasma pneumoniae: current status of diagnostic methods.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2010Review
- Microfluidic platform versus conventional real-time polymerase chain reaction for the detection of Mycoplasma pneumoniae in respiratory specimens.Diagnostic microbiology and infectious disease · 2010Article
- A low density oligonucleotide microarray for the detection of viral and atypical bacterial respiratory pathogens.Journal of virological methods · 2010Article
- Development and assessment of a multiplex real-time PCR assay for quantification of human immunodeficiency virus type 1 DNA.Journal of clinical microbiology · 2009Article
- The role of Mycoplasma in upper respiratory infections.Current infectious disease reports · 2009Article
- Comparison of commercial and in-house real-time PCR assays used for detection of Mycoplasma pneumoniae.Journal of clinical microbiology · 2009Article
- Evaluation of three real-time PCR assays for detection of Mycoplasma pneumoniae in an outbreak investigation.Journal of clinical microbiology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A real-time PCR was designed for detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae such that each pathogen could be detected in a single tube and differentiated using molecular beacons marked with different fluorochromes. This duplex PCR, targeting the P1 adhesion gene for M. pneumoniae and the ompA gene for C. pneumoniae, was compared with two conventional PCR assays targeting the 16S rRNA gene and the ompA gene. A total of 120 clinical throat and nasopharyngeal swab samples were tested. DNA extraction was performed using an alkali denaturation/neutralization method, and real-time amplification, detection, and data analysis were performed using a Rotor-Gene 2000 real-time rotary analyzer (Corbett Life Science, Sydney, Australia). Using conventional PCR as a reference in an analysis of 120 samples, 13 of 14 samples positive for C. pneumoniae were detected by the novel real-time PCR. In an analysis of M. pneumoniae, 22 samples were positive in the conventional PCR and the novel assay detected 24 positive samples. When using the conventional PCR as a reference, sensitivity and specificity were 93% and 100%, respectively, for C. pneumoniae and 100% and 98%, respectively, for M. pneumoniae. With an overall agreement of 98.8%, this suggests that performance of the new duplex real-time PCR is comparable to that of conventional PCR.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.