ArticleRNA (New York, N.Y.)2008
Puf1p acts in combination with other yeast Puf proteins to control mRNA stability.
Article in RNA (New York, N.Y.), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- PUF3 RNA binding protein ofHeliyon · 2024Article
- Regulatory activity is the default DNA state in eukaryotes.Nature structural & molecular biology · 2024Article
- Fusarium oxysporum f. sp.mBio · 2023Article
- Phosphorylation of mRNA-Binding Proteins Puf1 and Puf2 by TORC2-Activated Protein Kinase Ypk1 Alleviates Their Repressive Effects.Membranes · 2021Article
- Phase transition behaviour in yeast and bacterial populations under stress.Royal Society open science · 2020Article
- Regulation of Translation in the Protozoan ParasiteInternational journal of molecular sciences · 2020Review
- Distinct RNA-binding modules in a single PUF protein cooperate to determine RNA specificity.Nucleic acids research · 2019Article
- Article
- The Pumilio-domain protein PUF6 contributes to SIDER2 retroposon-mediated mRNA decay inRNA (New York, N.Y.) · 2017Article
- Upregulation of SPS100 gene expression by an antisense RNA via a switch of mRNA isoforms with different stabilities.Nucleic acids research · 2017Article
- Identification and characterization of roles for Puf1 and Puf2 proteins in the yeast response to high calcium.Scientific reports · 2017Article
- Integrated multi-omics analyses reveal the pleiotropic nature of the control of gene expression by Puf3p.Scientific reports · 2015Article
- RNA regulatory networks diversified through curvature of the PUF protein scaffold.Nature communications · 2015Article
- Glucose-Regulated Phosphorylation of the PUF Protein Puf3 Regulates the Translational Fate of Its Bound mRNAs and Association with RNA Granules.Cell reports · 2015Article
- Conditional regulation of Puf1p, Puf4p, and Puf5p activity alters YHB1 mRNA stability for a rapid response to toxic nitric oxide stress in yeast.Molecular biology of the cell · 2015Article
- Depletion of the Trypanosome Pumilio domain protein PUF2 or of some other essential proteins causes transcriptome changes related to coding region length.Eukaryotic cell · 2014Article
- Carbon source-dependent alteration of Puf3p activity mediates rapid changes in the stabilities of mRNAs involved in mitochondrial function.Nucleic acids research · 2014Article
- Alternative polyadenylation diversifies post-transcriptional regulation by selective RNA-protein interactions.Molecular systems biology · 2014Article
- Puf mediates translation repression of transmission-blocking vaccine candidates in malaria parasites.PLoS pathogens · 2013Article
- The trypanosome Pumilio domain protein PUF5.PloS one · 2013Article
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Authors and funding
2 authors.
Funding
Abstract
The eukaryotic Puf proteins bind 3' untranslated region (UTR) sequence elements to regulate the stability and translation of their target transcripts, and such regulatory events are critical for cell growth and development. Several global genome analyses have identified hundreds of potential mRNA targets of the Saccharomyces cerevisiae Puf proteins; however, only three mRNA targets for these proteins have been characterized thus far. After direct testing of nearly 40 candidate mRNAs, we established two of these as true mRNA targets of Puf-mediated decay in yeast, HXK1 and TIF1. In a novel finding, multiple Puf proteins, including Puf1p, regulate both of these mRNAs in combination. TIF1 mRNA decay can be stimulated individually by Puf1p and Puf5p, but the combination of both proteins is required for full regulation. This Puf-mediated decay requires the presence of two UGUA binding sites within the TIF1 3' UTR, with one site regulated by Puf5p and the other by both Puf1p and Puf5p. Alteration of the UGUA site in the tif1 3' UTR to more closely resemble the Puf3p binding site broadens the specificity to include regulation by Puf3p. The stability of the endogenously transcribed HXK1 mRNA, cellular levels of Hxk1 protein activity, and HXK1 3' UTR-directed decay are affected by Puf1p and Puf5p as well as Puf4p. Together these results identify the first mRNA targets of Puf1p-mediated decay, describe similar yet distinct combinatorial control of two new target mRNAs by the yeast Puf proteins, and suggest the importance of direct testing to evaluate RNA-regulatory mechanisms.
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