ArticleMolecular biology of the cell2008
A novel site of action for alpha-SNAP in the SNARE conformational cycle controlling membrane fusion.
Article in Molecular biology of the cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 49 citations in OpenAlex.
- ATPase N-ethylmaleimide-sensitive factor mediated calcium dependent fusion pore closure and endocytosis.Communications biology · 2026Article
- A pathogenic mutation in α-SNAP impairs membrane lipid binding by concealing a critical hydrophobic loop.Communications biology · 2026Article
- Evolutionary duplication of the leishmanial adaptor protein α-SNAP plays a role in its pathogenicity.The Journal of biological chemistry · 2025Article
- Detection of rare nematode resistance Rhg1 haplotypes in Glycine soja and a novel Rhg1 α-SNAP.The plant genome · 2022Article
- Dynamin controls neuropeptide secretion by organizing dense-core vesicle fusion sites.Science advances · 2021Article
- Munc18-1 is crucial to overcome the inhibition of synaptic vesicle fusion by αSNAP.Nature communications · 2019Article
- Sec17 (α-SNAP) and an SM-tethering complex regulate the outcome of SNARE zippering in vitro and in vivo.eLife · 2017Article
- Components of the SNARE-containing regulon are co-regulated in root cells undergoing defense.Plant signaling & behavior · 2017Article
- AFM/TIRF force clamp measurements of neurosecretory vesicle tethers reveal characteristic unfolding steps.PloS one · 2017Article
- Disease resistance through impairment of α-SNAP-NSF interaction and vesicular trafficking by soybean Rhg1.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- A Highly Polymorphic Copy Number Variant in the NSF Gene is Associated with Cocaine Dependence.Scientific reports · 2016Article
- α-SNAP interferes with the zippering of the SNARE protein membrane fusion machinery.The Journal of biological chemistry · 2014Article
- Syntaxin 31 functions in Glycine max resistance to the plant parasitic nematode Heterodera glycines.Plant molecular biology · 2014Article
- Article
- Crystal structure of the human short coiled coil protein and insights into SCOC-FEZ1 complex formation.PloS one · 2013Article
- Controlling synaptotagmin activity by electrostatic screening.Nature structural & molecular biology · 2012Article
- The expression of a naturally occurring, truncated allele of an α-SNAP gene suppresses plant parasitic nematode infection.Plant molecular biology · 2012Article
- Requirements for the catalytic cycle of the N-ethylmaleimide-Sensitive Factor (NSF).Biochimica et biophysica acta · 2012Review
- SNARE-mediated rapid lysosome fusion in membrane raft clustering and dysfunction of bovine coronary arterial endothelium.American journal of physiology. Heart and circulatory physiology · 2011Article
- Ca2+ induces clustering of membrane proteins in the plasma membrane via electrostatic interactions.The EMBO journal · 2011Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Regulated exocytosis in neurons and neuroendocrine cells requires the formation of a stable soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex consisting of synaptobrevin-2/vesicle-associated membrane protein 2, synaptosome-associated protein of 25 kDa (SNAP-25), and syntaxin 1. This complex is subsequently disassembled by the concerted action of alpha-SNAP and the ATPases associated with different cellular activities-ATPase N-ethylmaleimide-sensitive factor (NSF). We report that NSF inhibition causes accumulation of alpha-SNAP in clusters on plasma membranes. Clustering is mediated by the binding of alpha-SNAP to uncomplexed syntaxin, because cleavage of syntaxin with botulinum neurotoxin C1 or competition by using antibodies against syntaxin SNARE motif abolishes clustering. Binding of alpha-SNAP potently inhibits Ca(2+)-dependent exocytosis of secretory granules and SNARE-mediated liposome fusion. Membrane clustering and inhibition of both exocytosis and liposome fusion are counteracted by NSF but not when an alpha-SNAP mutant defective in NSF activation is used. We conclude that alpha-SNAP inhibits exocytosis by binding to the syntaxin SNARE motif and in turn prevents SNARE assembly, revealing an unexpected site of action for alpha-SNAP in the SNARE cycle that drives exocytotic membrane fusion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.