ArticleParasitology research2008
Characterization of cysteine proteases from the carcinogenic liver fluke, Opisthorchis viverrini.
Article in Parasitology research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Trematode Genomics and Proteomics.Advances in experimental medicine and biology · 2024Article
- Cystatins from the Human Liver FlukePathogens (Basel, Switzerland) · 2023Article
- Exploring the role of macrophages in determining the pathogenesis of liver fluke infection.Parasitology · 2022Review
- Cysteine proteases during larval migration and development of helminths in their final host.PLoS neglected tropical diseases · 2018Review
- Opisthorchis viverrini Proteome and Host-Parasite Interactions.Advances in parasitology · 2018Review
- Molecular and Biochemical Characterization of Opisthorchis viverrini Calreticulin.The Korean journal of parasitology · 2017Article
- Inhibition of cathepsin B activity by 2,3,7,8-tetrachlorodibenzo-p-dioxin.Environmental science and pollution research international · 2015Article
- Cysteine protease is a major component in the excretory/secretory products of Euclinostomum heterostomum (Digenea: Clinostomidae).Parasitology research · 2014Article
- Molecular characterization and expression of a cysteine protease from Clonorchis sinensis and its application for serodiagnosis of clonorchiasis.Parasitology research · 2012Article
- Significance of Cuscutain, a cysteine protease from Cuscuta reflexa, in host-parasite interactions.BMC plant biology · 2010Article
- An update on metacercarial excystment of trematodes.Parasitology research · 2009Review
- Utility of a protein fraction with cathepsin L-Like activity purified from cysticercus fluid of Taenia solium in the diagnosis of human cysticercosis.The American journal of tropical medicine and hygiene · 2009Article
- Ov-APR-1, an aspartic protease from the carcinogenic liver fluke, Opisthorchis viverrini: functional expression, immunolocalization and subsite specificity.The international journal of biochemistry & cell biology · 2009Article
- Cathepsin F cysteine protease of the human liver fluke, Opisthorchis viverrini.PLoS neglected tropical diseases · 2009Article
- Asparaginyl endopeptidase from the carcinogenic liver fluke, Opisthorchis viverrini, and its potential for serodiagnosis.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2008Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
Protease activities in extracts of Opisthorchis viverrini were investigated using gelatin zymography and fluorogenic peptide substrates. Using gelatin-impregnated X-ray film, 2 microg of O. viverrini excretory-secretory products (Ov-ES) and adult somatic extract (Ov-SE) showed proteolytic activity. Zymography of both O. viverrini extracts revealed bands at approximately 30 kDa. Using fluorogenic peptide substrates, the majority of O. viverrini activity was determined to be cathepsin L-like cysteine protease (cleaved Z-Phe-Arg-aminomethylcoumarin (AMC)) whereas little or no activity was ascribable to other classes of proteases. The O. viverrini cysteine protease activity was greatest at pH 6.0 and the activity was inhibited by the class-specific inhibitors, E-64 and Z-Ala-CHN2. Chromatographic purification of O. viverrini cysteine proteases on thiol-sepharose enriched for protein(s) of approximately 30 kDa from Ov-ES and Ov-SE. The activity profile of the purified enzyme was similar to that of the cathepsin L-like activity characterized in Ov-SE and Ov-ES. Furthermore, determination of cysteine protease activity in several developmental stages of the parasite revealed the highest protease activity in metacercariae soluble extract, followed by Ov-ES, egg soluble extract, and Ov-SE. These findings demonstrated that O. viverrini has a cathepsin L-like cysteine protease(s) and suggested that abundant cysteine protease activity was present in metacercariae where the hydrolase might be involved in cyst excystation during mammalian infection.
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