ArticleThe Journal of general virology2008
Functional analysis of human cytomegalovirus pUS28 mutants in infected cells.
Article in The Journal of general virology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- The human cytomegalovirus vGPCR UL33 is essential for efficient lytic replication in epithelial cells.Journal of virology · 2026Article
- Delivery of US28 by incoming HCMV particles rapidly attenuates Akt activity to suppress HCMV lytic replication in monocytes.Science signaling · 2024Article
- Cytomegalovirus US28 regulates cellular EphA2 to maintain viral latency.Science advances · 2022Article
- Optimization of a Lambda-RED Recombination Method for Rapid Gene Deletion in Human Cytomegalovirus.International journal of molecular sciences · 2021Article
- Methods for Studying the Function of Cytomegalovirus GPCRs.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The constitutive activity of the viral-encoded G protein-coupled receptor US28 supports a complex signalling network contributing to cancer development.Biochemical Society transactions · 2020Review
- Attenuation of chemokine receptor function and surface expression as an immunomodulatory strategy employed by human cytomegalovirus is linked to vGPCR US28.Cell communication and signaling : CCS · 2016Article
- The HCMV US28 vGPCR induces potent Gαq/PLC-β signaling in monocytes leading to increased adhesion to endothelial cells.Virology · 2016Article
- Human Cytomegalovirus US28 Is Important for Latent Infection of Hematopoietic Progenitor Cells.Journal of virology · 2015Article
- Modulation of cellular signaling by herpesvirus-encoded G protein-coupled receptors.Frontiers in pharmacology · 2015Review
- Article
- Identification of common mechanisms by which human and mouse cytomegalovirus seven-transmembrane receptor homologues contribute to in vivo phenotypes in a mouse model.Journal of virology · 2013Article
- US28 is a potent activator of phospholipase C during HCMV infection of clinically relevant target cells.PloS one · 2012Article
- The carboxy-terminal tail of human cytomegalovirus (HCMV) US28 regulates both chemokine-independent and chemokine-dependent signaling in HCMV-infected cells.Journal of virology · 2009Article
- Activation of intracellular signaling pathways by the murine cytomegalovirus G protein-coupled receptor M33 occurs via PLC-{beta}/PKC-dependent and -independent mechanisms.Journal of virology · 2009Article
- Feeling manipulated: cytomegalovirus immune manipulation.Virology journal · 2009Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The human cytomegalovirus (HCMV)-encoded viral G protein-coupled receptor pUS28 contributes to an array of biological effects, including cell migration and proliferation. Using FIX-BAC (bacterial artificial chromosome, derived from the HCMV clinical isolate VR1814) and lambda red recombination techniques, we generated HCMV recombinants expressing amino-terminally FLAG-tagged versions of wild-type pUS28 (FLAG-US28/WT), G-protein coupling deficient pUS28 (FLAG-US28/R129A) and chemokine-binding domain deficient pUS28 (FLAG-US28/DeltaN). Infection with the FLAG-US28/R129A virus failed to induce inositol phosphate accumulation, indicating that G-protein coupling is essential for pUS28 signalling to phospholipase C-beta (PLC-beta) during HCMV infection. The FLAG-US28/DeltaN virus induced about 80 % of the level of PLC-beta signalling induced by the FLAG-US28/WT virus, demonstrating that the N-terminal chemokine-binding domain is not required for pUS28-induced PLC-beta signalling in infected cells. The data presented here are the first to describe the functional analyses of several key pUS28 mutants in HCMV-infected cells. Elucidating the mechanisms by which pUS28 signals during infection will provide important insights into HCMV pathogenesis.
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