ArticleThe Journal of biological chemistry2008
Immediate-early signaling induced by E-cadherin engagement and adhesion.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed.
- StretchView - A Multi-Axial Cell-Stretching Device for Long-Term Automated Videomicroscopy of Living Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- An actin bracket-induced elastoplastic transition determines epithelial folding irreversibility.Nature communications · 2024Article
- 3D cell segregation geometry and dynamics are governed by tissue surface tension regulation.Communications biology · 2023Article
- Transient interactions drive the lateral clustering of cadherin-23 on membrane.Communications biology · 2023Article
- Adhesion-Based Self-Organization in Tissue Patterning.Annual review of cell and developmental biology · 2022Review
- Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion in zebrafish germ-layer progenitor cells.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A Stiff Extracellular Matrix Favors the Mechanical Cell Competition that Leads to Extrusion of Bacterially-Infected Epithelial Cells.Frontiers in cell and developmental biology · 2022Article
- Volume measurement and biophysical characterization of mounds in epithelial monolayers after intracellular bacterial infection.STAR protocols · 2021Article
- Mechanical competition triggered by innate immune signaling drives the collective extrusion of bacterially infected epithelial cells.Developmental cell · 2021Article
- FluoSim: simulator of single molecule dynamics for fluorescence live-cell and super-resolution imaging of membrane proteins.Scientific reports · 2020Article
- Non-junctional role of Cadherin3 in cell migration and contact inhibition of locomotion via domain-dependent, opposing regulation of Rac1.Scientific reports · 2020Article
- Recent Advances and Prospects in the Research of Nascent Adhesions.Frontiers in physiology · 2020Review
- Probing force in living cells with optical tweezers: from single-molecule mechanics to cell mechanotransduction.Biophysical reviews · 2019Review
- Statistical Mechanics of an Elastically Pinned Membrane: Equilibrium Dynamics and Power Spectrum.Biophysical journal · 2019Article
- Statistical Mechanics of an Elastically Pinned Membrane: Static Profile and Correlations.Biophysical journal · 2019Article
- Engineering small tubes with changes in diameter for the study of kidney cell organization.Biomicrofluidics · 2018Article
- The WAVE Regulatory Complex and Branched F-Actin Counterbalance Contractile Force to Control Cell Shape and Packing in the Drosophila Eye.Developmental cell · 2018Article
- Changes in E-cadherin rigidity sensing regulate cell adhesion.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Actin-Based Adhesion Modules Mediate Cell Interactions with the Extracellular Matrix and Neighboring Cells.Cold Spring Harbor perspectives in biology · 2017Review
- Non-canonical functions of claudin proteins: Beyond the regulation of cell-cell adhesions.Tissue barriers · 2017Review
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Epithelial cell-cell interactions require localized adhesive interactions between E-cadherin on opposing membranes and the activation of downstream signaling pathways that affect membrane and actin dynamics. However, it is not known whether E-cadherin engagement and activation of these signaling pathways are locally coordinated or whether signaling is sustained or locally down-regulated like other receptor-mediated pathways. To obtain high spatiotemporal resolution of immediate-early signaling events upon E-cadherin engagement, we used laser tweezers to place beads coated with functional E-cadherin extracellular domain on cells. We show that cellular E-cadherin accumulated rapidly around beads, reaching a sustained plateau level in 1-3 min. Phosphoinositides and Rac1 co-accumulated with E-cadherin, reached peak levels with E-cadherin, but then rapidly dispersed. Both E-cadherin and Rac1 accumulated independently of Rac1 GTP binding/hydrolysis, but these activities were required for Rac1 dispersal. E-cadherin accumulation was dependent on membrane dynamics and actin polymerization, but actin did not stably co-accumulate with E-cadherin; mathematical modeling showed that diffusion-mediated trapping could account for the initial E-cadherin accumulation. We propose that initial E-cadherin accumulation requires active membrane dynamics and involves diffusion-mediated trapping at contact sites; to propagate further contacts, phosphatidylinositol 3-kinase and Rac1 are transiently activated by E-cadherin engagement and initiate a new round of membrane dynamics, but they are subsequently suppressed at that site to allow maintenance of weak E-cadherin mediated adhesion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.