ArticleBMC biotechnology2007
Microarray-based method for detection of unknown genetic modifications.
Article in BMC biotechnology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- A multi-channel CRISPR-based method for rapid, sensitive detection of four diseases ofHorticulture research · 2025Article
- Review of CRISPR/Cas Systems on Detection of Nucleotide Sequences.Foods (Basel, Switzerland) · 2023Review
- Detection and identification of transgenic events by next generation sequencing combined with enrichment technologies.Scientific reports · 2019Article
- Discrimination of Transgenic Maize Kernel Using NIR Hyperspectral Imaging and Multivariate Data Analysis.Sensors (Basel, Switzerland) · 2017Article
- Characterization of unknown genetic modifications using high throughput sequencing and computational subtraction.BMC biotechnology · 2009Article
- Optimised padlock probe ligation and microarray detection of multiple (non-authorised) GMOs in a single reaction.BMC genomics · 2008Article
- NAIMA: target amplification strategy allowing quantitative on-chip detection of GMOs.Nucleic acids research · 2008Article
Corrections and comments
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Authors and funding
7 authors.
Funding
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Abstract
backgroundDue to the increased use of genetic modifications in crop improvement, there is a need to develop effective methods for the detection of both known and unknown transgene constructs in plants. We have developed a strategy for detection and characterization of unknown genetic modifications and we present a proof of concept for this method using Arabidopsis thaliana and Oryza sativa (rice). The approach relies on direct hybridization of total genomic DNA to high density microarrays designed to have probes tiled throughout a set of reference sequences.
resultsWe show that by using arrays with 25 basepair probes covering both strands of a set of 235 vectors (2 million basepairs) we can detect transgene sequences in transformed lines of A. thaliana and rice without prior knowledge about the transformation vectors or the T-DNA constructs used to generate the studied plants.
conclusionThe approach should allow the user to detect the presence of transgene sequences and get sufficient information for further characterization of unknown genetic constructs in plants. The only requirements are access to a small amount of pure transgene plant material, that the genetic construct in question is above a certain size (here >/= 140 basepairs) and that parts of the construct shows some degree of sequence similarity with published genetic elements.
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