ArticleParasitology research2008
Cloning and characterization of a cDNA clone encoding calreticulin from Haemaphysalis qinghaiensis (Acari: Ixodidae).
Article in Parasitology research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Distribution and diversity of ticks determined by environmental factors in Ningxia, China.One health (Amsterdam, Netherlands) · 2024Article
- The effect of feeding on different hosts on the egg proteins in Haemaphysalis qinghaiensis tick.Parasitology research · 2024Article
- Changes in saliva protein profile throughout Rhipicephalus microplus blood feeding.Parasites & vectors · 2024Article
- Tick Vaccines and Concealed versus Exposed Antigens.Pathogens (Basel, Switzerland) · 2023Article
- High prevalence of spotted fever group rickettsiae in ticks collected from yaks (Frontiers in microbiology · 2022Article
- Vertebrate Responses against Arthropod Salivary Proteins and Their Therapeutic Potential.Vaccines · 2021Review
- Spotted Fever GroupFrontiers in veterinary science · 2021Article
- Molecular detection of Bartonella in ixodid ticks collected from yaks and plateau pikas (Ochotona curzoniae) in Shiqu County, China.BMC veterinary research · 2020Article
- Identification ofPathogens (Basel, Switzerland) · 2020Article
- The Interactions of Parasite Calreticulin With Initial Complement Components: Consequences in Immunity and Virulence.Frontiers in immunology · 2020Review
- Exon-intron structure and sequence variation of the calreticulin gene among Rhipicephalus sanguineus group ticks.Parasites & vectors · 2016Article
- Molecular survey and characterization of a novel Anaplasma species closely related to Anaplasma capra in ticks, northwestern China.Parasites & vectors · 2016Article
- Novel spotted fever group rickettsiae in Haemaphysalis qinghaiensis ticks from Gansu, Northwest China.Parasites & vectors · 2016Article
- Constructing and detecting a cDNA library for mites.Parasitology research · 2015Article
- Amblyomma americanum tick calreticulin binds C1q but does not inhibit activation of the classical complement cascade.Ticks and tick-borne diseases · 2015Article
- Proteomic screening of antigenic proteins from the hard tick, Haemaphysalis longicornis (Acari: Ixodidae).The Korean journal of parasitology · 2015Article
- A Nematode Calreticulin, Rs-CRT, Is a Key Effector in Reproduction and Pathogenicity of Radopholus similis.PloS one · 2015Article
- Susceptibility of the tick Haemaphysalis qinghaiensis to isolates of the fungus Metarhizium anisopliae in China.Experimental & applied acarology · 2014Article
- Deorphanization and target validation of cross-tick species conserved novel Amblyomma americanum tick saliva protein.International journal for parasitology · 2013Article
- The life cycle of Haemaphysalis qinghaiensis (Acari: Ixodidae) ticks under laboratory conditions.Experimental & applied acarology · 2013Article
Corrections and comments
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The application of anti-tick vaccine has been shown to be the most promising alternative strategy compared to the current use of acaricides that suffer from a number of serious limitations. The success of this method is dependent upon identification and cloning of potential tick vaccine antigens. Previously, we have cloned 21 positive clones (named from Hq02 to Hq22) by immunoscreening complimentary DNA (cDNA) libraries of Haemaphysalis qinghaiensis; however, some of those clones did not contain open reading frames (ORF). In this study, we amplified the entire sequence of Hq07 by using rapid amplification of the cDNA ends. Hq07 contains an ORF of 1,233 bp that encodes for 410 amino acid residues with a coding capacity of 47 kDa. Search of the cloned sequences against GenBank revealed that Hq07 is a calreticulin (CRT)-similar clone and designated HqCRT. Expression analysis by reverse transcription-polymerase chain reaction showed that this gene is ubiquitously expressed at different developmental stages and in different tissues of H. qinghaiensis. The gene was expressed as glutathione S-transferase-fused proteins in a prokaryotic system. Western blot analysis revealed that native HqCRT was secreted into their hosts by ticks during blood sucking. Vaccination of sheep with rHqCRT conferred protective immunity against ticks, resulting in 54.3% mortality in adult ticks, compared to the 38.7% death rate in the control group. These results demonstrated that rHqCRT might be a useful vaccine candidate antigen for biological control of H. qinghaiensis.
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