ArticleExtremophiles : life under extreme conditions2008
Lysis efficiency of standard DNA extraction methods for Halococcus spp. in an organic rich environment.
Article in Extremophiles : life under extreme conditions, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.
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- Decoding extremophiles: insights from bioinformatics, machine learning, and data-driven approaches.Briefings in bioinformatics · 2026Review
- Production, characterization, and genomic insights of a human lectin-binding exopolysaccharide from Haloarcula japonica strain SST1.Scientific reports · 2025Article
- Life in the brine of Lunenburg, Germany: unveiling microorganisms associated with Zechstein salt deposits.Frontiers in microbiology · 2025Article
- Establishment of a consensus protocol to explore the brain pathobiome in patients with mild cognitive impairment and Alzheimer's disease: Research outline and call for collaboration.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2023Review
- Direct capture and amplification of nucleic acids using a universal, elution-free magnetic bead-based method for rapid pathogen detection in multiple types of biological samples.Analytical and bioanalytical chemistry · 2023Article
- Nucleic acid extraction without electrical equipment via magnetic nanoparticles in Pasteur pipettes for pathogen detection.Analytical biochemistry · 2021Article
- An Improved Method for the Extraction of Nucleic Acids from Plant Tissue without Grinding to Detect Plant Viruses and Viroids.Plants (Basel, Switzerland) · 2021Article
- Duplication and diversification of a unique chromosomal virulence island hosting the subtilase cytotoxin inMicrobial genomics · 2020Article
- Infrared Spectroscopic Detection of Biosignatures at Lake Tírez, Spain: Implications for Mars.Astrobiology · 2020Article
- Molecular Ecology of Hypersaline Microbial Mats: Current Insights and New Directions.Microorganisms · 2016Review
- Polymerase chain reaction-based screening method applicable universally to environmental haloarchaea and halobacteria for identifying polyhydroxyalkanoate producers among them.Extremophiles : life under extreme conditions · 2015Article
- Adaptation, ecology, and evolution of the halophilic stromatolite archaeon Halococcus hamelinensis inferred through genome analyses.Archaea (Vancouver, B.C.) · 2015Article
- Comparative study of fungal cell disruption--scope and limitations of the methods.Folia microbiologica · 2011Article
- Occurrence of Halococcus spp. in the nostrils salt glands of the seabird Calonectris diomedea.Extremophiles : life under extreme conditions · 2009Article
- A comparison of methods for total community DNA preservation and extraction from various thermal environments.Journal of industrial microbiology & biotechnology · 2008Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The extraction of nucleic acids from a given environment marks a crucial and essential starting point in any molecular investigation. Members of Halococcus spp. are known for their rigid cell walls, and are thus difficult to lyse and could potentially be overlooked in an environment. Furthermore, the lack of a suitable lysis method hinders subsequent molecular analysis. The effects of six different DNA extraction methods were tested on Halococcus hamelinensis, Halococcus saccharolyticus and Halobacterium salinarum NRC-1 as well as on an organic rich, highly carbonated sediment from stromatolites spiked with Halococcus hamelinensis. The methods tested were based on physical disruption (boiling and freeze/thawing), chemical lysis (Triton X-100, potassium ethyl xanthogenate (XS) buffer and CTAB) and on enzymatic lysis (lysozyme). Results showed that boiling and freeze/thawing had little effect on the lysis of both Halococcus strains. Methods based on chemical lysis (Triton X-100, XS-buffer, and CTAB) showed the best results, however, Triton X-100 treatment failed to produce visible DNA fragments. Using a combination of bead beating, chemical lysis with lysozyme, and thermal shock, lysis of cells was achieved however DNA was badly sheared. Lysis of cells and DNA extraction of samples from spiked sediment proved to be difficult, with the XS-buffer method indicating the best results. This study provides an evaluation of six commonly used methods of cell lysis and DNA extraction of Halococcus spp., and the suitability of the resulting DNA for molecular analysis.
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