ArticleMolecular and cellular biology2008
Slx5 promotes transcriptional silencing and is required for robust growth in the absence of Sir2.
Article in Molecular and cellular biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 27 citations in OpenAlex.
- Recruitment of a SUMO isopeptidase to rDNA stabilizes silencing complexes by opposing SUMO targeted ubiquitin ligase activity.Genes & development · 2017Article
- Identification of Components of the SUMOylation Machinery in Candida glabrata: ROLE OF THE DESUMOYLATION PEPTIDASE CgUlp2 IN VIRULENCE.The Journal of biological chemistry · 2016Article
- SUMO Pathway Modulation of Regulatory Protein Binding at the Ribosomal DNA Locus in Saccharomyces cerevisiae.Genetics · 2016Article
- Functions for diverse metabolic activities in heterochromatin.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Sumoylation of Sir2 differentially regulates transcriptional silencing in yeast.Nucleic acids research · 2015Article
- Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.Nature communications · 2015Article
- A moonlighting metabolic protein influences repair at DNA double-stranded breaks.Nucleic acids research · 2015Article
- Article
- STUbLs in chromatin and genome stability.Biopolymers · 2013Review
- SUMO: a multifaceted modifier of chromatin structure and function.Developmental cell · 2013Review
- Centromere binding and a conserved role in chromosome stability for SUMO-dependent ubiquitin ligases.PloS one · 2013Article
- Yeast Tdh3 (glyceraldehyde 3-phosphate dehydrogenase) is a Sir2-interacting factor that regulates transcriptional silencing and rDNA recombination.PLoS genetics · 2013Article
- Functional diversification of yeast telomere associated protein, Rif1, in higher eukaryotes.BMC genomics · 2012Article
- The SUMO E3 ligase Siz2 exerts a locus-dependent effect on gene silencing in Saccharomyces cerevisiae.Eukaryotic cell · 2012Article
- Functional antagonism between Sas3 and Gcn5 acetyltransferases and ISWI chromatin remodelers.PLoS genetics · 2012Article
- Genetic analysis implicates the Set3/Hos2 histone deacetylase in the deposition and remodeling of nucleosomes containing H2A.Z.Genetics · 2011Article
- RING domain dimerization is essential for RNF4 function.The Biochemical journal · 2010Article
- The glucanosyltransferase Gas1 functions in transcriptional silencing.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Genome stability roles of SUMO-targeted ubiquitin ligases.DNA repair · 2009Review
- The SUMO-targeted ubiquitin ligase subunit Slx5 resides in nuclear foci and at sites of DNA breaks.Cell cycle (Georgetown, Tex.) · 2009Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The broadly conserved Sir2 NAD(+)-dependent deacetylase is required for chromatin silencing. Here we report the discovery of physical and functional links between Sir2 and Slx5 (Hex3), a RING domain protein and subunit of the Slx5/8 complex, [corrected] which is a ubiquitin E3 ligase that targets sumoylated proteins. Slx5 interacted with Sir2 by two-hybrid and glutathione S-transferase-binding assays and was found to promote silencing of genes at telomeric or ribosomal DNA (rDNA) loci. However, deletion of SLX5 had no detectable effect on the distribution of silent chromatin components and only slightly altered the deacetylation of histone H4 lysine 16 at the telomere. In vivo assays indicated that Sir2-dependent silencing was functionally intact in the absence of Slx5. Although no previous reports suggest that Sir2 contributes to the fitness of yeast populations, we found that Sir2 was required for maximal growth in slx5Delta mutant cells. A similar requirement was observed for mutants of the SUMO isopeptidase Ulp2/Smt4. The contribution of Sir2 to optimal growth was not due to known Sir2 roles in mating-type determination or rDNA maintenance but was connected to a role of sumoylation in transcriptional silencing. These results indicate that Sir2 and Slx5 jointly contribute to transcriptional silencing and robust cellular growth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.