ArticleNucleic acids research2008
Structure-function analysis of the RNA helicase maleless.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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Who cites it
28 citing papers in PubMed, 48 citations in OpenAlex.
- Nucleotide-Resolution Mapping Reveals Specific MLE Binding Site on roX2 lncRNA.Journal of molecular biology · 2026Article
- Evolutionarily Conserved DHX9/MLE Helicase Is Involved in the Regulation of Its Own mRNA Expression Level in Drosophila melanogaster.Doklady. Biochemistry and biophysics · 2025Article
- DHX9 SUMOylation is required for the suppression of R-loop-associated genome instability.Nature communications · 2024Article
- Interaction of MLE with CLAMP zinc finger is involved in proper MSL proteins binding to chromosomes inOpen biology · 2024Article
- Regulation and mechanisms of action of RNA helicases.RNA biology · 2024Review
- Divergent evolution toward sex chromosome-specific gene regulation inGenes & development · 2021Article
- Identification of Intrinsic RNA Binding Specificity of Purified Proteins byBio-protocol · 2021Article
- Two-step mechanism for selective incorporation of lncRNA into a chromatin modifier.Nucleic acids research · 2020Article
- Progressive dosage compensation during Drosophila embryogenesis is reflected by gene arrangement.EMBO reports · 2019Article
- Structure, dynamics and roX2-lncRNA binding of tandem double-stranded RNA binding domains dsRBD1,2 of Drosophila helicase Maleless.Nucleic acids research · 2019Article
- Structural insights reveal the specific recognition of roX RNA by the dsRNA-binding domains of the RNA helicase MLE and its indispensable role in dosage compensation in Drosophila.Nucleic acids research · 2019Article
- Protein features for assembly of the RNA editing helicase 2 subcomplex (REH2C) in Trypanosome holo-editosomes.PloS one · 2019Article
- How do lncRNAs regulate transcription?Science advances · 2017Review
- The landscape of DNA methylation-mediated regulation of long non-coding RNAs in breast cancer.Oncotarget · 2017Article
- Article
- The Drosophila Helicase Maleless (MLE) is Implicated in Functions Distinct From its Role in Dosage Compensation.Molecular & cellular proteomics : MCP · 2015Article
- A new player in X identification: the CLAMP protein is a key factor in Drosophila dosage compensation.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2014Review
- Are we there yet? Initial targeting of the Male-Specific Lethal and Polycomb group chromatin complexes in Drosophila.Open biology · 2014Review
- Tandem stem-loops in roX RNAs act together to mediate X chromosome dosage compensation in Drosophila.Molecular cell · 2013Article
- Chromatin proteins captured by ChIP-mass spectrometry are linked to dosage compensation in Drosophila.Nature structural & molecular biology · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Loss of function of the RNA helicase maleless (MLE) in Drosophila melanogaster leads to male-specific lethality due to a failure of X chromosome dosage compensation. MLE is presumably involved in incorporating the non-coding roX RNA into the dosage compensation complex (DCC), which is an essential but poorly understood requirement for faithful targeting of the complex to the X chromosome. Sequence comparison predicts several RNA-binding domains in MLE but their properties have not been experimentally verified. We evaluated the RNA-binding characteristics of these conserved motifs and their contributions to RNA-stimulated ATPase activity, to helicase activity, as well as to the targeting of MLE to the nucleus and to the X chromosome territory. We find that RB2 is the dominant, conditional RNA-binding module, which is indispensable for ATPase and helicase activity whereas the N-terminal RB1 motif does not bind RNA, but is involved in targeting MLE to the X chromosome. The C-terminal domain containing a glycine-rich heptad repeat adds potential dimerization and RNA-binding surfaces which are not required for helicase activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.