ArticleNucleic acids research2008
Red flag on the white reporter: a versatile insulator abuts the white gene in Drosophila and is omnipresent in mini-white constructs.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Naturally Occurring Variability in the Regulatory Region of theInternational journal of molecular sciences · 2026Article
- Chromatin insulators homie and nhomie can interact with distant copies either together or separately, with distinct outcomes for enhancer-promoter interactions.PLoS genetics · 2026Article
- Article
- 3D chromatin structures precede genome activation in Drosophila embryogenesis.Cell genomics · 2025Article
- Article
- Demonstration of in vivo engineered tandem duplications of varying sizes using CRISPR and recombinases in Drosophila melanogaster.G3 (Bethesda, Md.) · 2023Article
- Context-dependent role of Pho binding sites in Polycomb complex recruitment in Drosophila.Genetics · 2023Article
- Demonstration ofbioRxiv : the preprint server for biology · 2023Article
- No apparent role for the Wari insulator in transcriptional regulation of the endogenousmicroPublication biology · 2023Article
- Boundaries potentiate polycomb response element-mediated silencing.BMC biology · 2021Article
- The Role of Insulators in Transgene Transvection inGenetics · 2019Article
- Biogenesis of zinc storage granules inThe Journal of experimental biology · 2018Article
- The X-linked 1.688 Satellite inGenetics · 2018Article
- Boundaries of loop domains (insulators): Determinants of chromosome form and function in multicellular eukaryotes.BioEssays : news and reviews in molecular, cellular and developmental biology · 2017Review
- Article
- Transcription through enhancers suppresses their activity in Drosophila.Epigenetics & chromatin · 2013Article
- Effective blocking of the white enhancer requires cooperation between two main mechanisms suggested for the insulator function.PLoS genetics · 2013Article
- New properties of Drosophila scs and scs' insulators.PloS one · 2013Article
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- Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Much of the research on insulators in Drosophila has been done with transgenic constructs using the white gene (mini-white) as reporter. Hereby we report that the sequence between the white and CG32795 genes in Drosophila melanogaster contains an insulator of a novel kind. Its functional core is within a 368 bp segment almost contiguous to the white 3'UTR, hence we name it as Wari (white-abutting resident insulator). Though Wari contains no binding sites for known insulator proteins and does not require Su(Hw) or Mod(mdg4) for its activity, it can equally well interact with another copy of Wari and with unrelated Su(Hw)-dependent insulators, gypsy or 1A2. In its natural downstream position, Wari reinforces enhancer blocking by any of the three insulators placed between the enhancer and the promoter; again, Wari-Wari, Wari-gypsy or 1A2-Wari pairing results in mutual neutralization (insulator bypass) when they precede the promoter. The distressing issue is that this element hides in all mini-white constructs employed worldwide to study various insulators and other regulatory elements as well as long-range genomic interactions, and its versatile effects could have seriously influenced the results and conclusions of many works.
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